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首页> 外文期刊>Plant and Soil >Nitric oxide mediates aluminum-induced citrate secretion through regulating the metabolism and transport of citrate in soybean roots
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Nitric oxide mediates aluminum-induced citrate secretion through regulating the metabolism and transport of citrate in soybean roots

机译：通过调节大豆根部的代谢和柠檬酸盐的代谢和运输，一氧化氮介导铝诱导的柠檬酸盐分泌

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AimsCitrate secretion is a kind of typical strategy for plant against aluminum (Al) toxicity. However, the signaling process in Al-activated citrate secretion needs to be clarified.MethodsPhysiological and biochemical methods as well as gene expression analysis were employed to examine the regulatory roles of nitric oxide (NO) in Al-activated citrate secretion in soybean roots.ResultsApplication of NO donor alleviated root growth inhibition and decreased Al content in Al-treated root apices. Al-induced NO production and citrate secretion were further elevated by NO donor, but inhibited by NO scavenger. Inhibition of citrate synthase (CS) or plasma membrane (PM) H+-ATPase activity significantly decreased Al-induced secretion of citrate, but inhibition of aconitase (ACO) activity enhanced citrate secretion under Al stress. Furthermore, NO mediated Al-stimulated CS and PM H+-ATPase activities, but decreased ACO activity under Al stress. Further investigation showed that NO modulated Al-activated transcriptional expression of CS and PM H+-ATPase as well as GmMATE. Overexpression of GmMATE in soybean hairy roots caused an enhanced Al-induced citrate efflux and Al resistance.ConclusionsOur findings suggest that NO-dependent up-regulation of citrate synthesis and activation of PM H+-ATPase-coupled MATE transporter co-transport system participates in Al-activated citrate exudation, thus conferring plant resistance to Al toxicity.
机译：Aimscitrate分泌是一种促进铝（Al）毒性的典型策略。然而，需要澄清Al活化的柠檬酸盐分泌中的信号传导过程。用过的物理学和生化方法以及基因表达分析，以检查大豆根系中的氧化氢（NO）在大豆根系中的柠檬酸盐分泌中的调节作用。方法没有施主减轻根系生长抑制和降低的Al处理的根部占子中的Al含量。 Al-诱导没有生产和柠檬酸盐分泌不受任何供体进一步升高，但不受清除剂抑制。抑制柠檬酸盐合酶（Cs）或血浆膜（PM）H + -ATP酶活性显着降低了柠檬酸盐的分泌显着降低，但抑制Aconitase（ACO）活性增强了Al胁迫下的柠檬酸盐分泌。此外，没有介导的Al刺激的Cs和PM H + -ATP酶活性，但在Al胁迫下降低ACO活性。进一步的研究表明，没有CS和PM H + -ATPase以及GEMATE的调节al-活化的转录表达。在大豆毛状根中过表达导致GMMate引起了增强的柠檬酸盐流速和Al抗性。结合调查结果表明，无依赖性上调柠檬酸盐合成和PM H + -ATPase耦合的配偶转运系统的激活参与Al - 激活的柠檬酸盐渗出，从而赋予植物抗性毒性。

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