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Developmental characteristics of grapevine seedlings root border cells and their response to rho-hydroxybenzoic acid

机译:葡萄幼苗根边界细胞的发育特征及其对rho-羟基苯甲酸的反应

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Aims rho-hydroxybenzoic acid (4-HBA) is one of the major autotoxins that cause grapevine replant disease. Root border cells (RBCs) play an important role in plant defense. Although RBCs have been investigated in many plants, relevant information in grapevines is limited. In this study, we aimed to investigate the developmental characteristics of RBCs in the grapevine (Vitis riparia x Vitis labrusca) 'Beta' and their response to 4-HBA stress. Methods RBCs were observed under a laser scanning confocal microscope. The phenolic acid composition of RBCs slime was determined by high-performance liquid chromatography. The level of gene expression was determined by quantitative real-time polymerase chain reaction. Results Grapevine RBCs have high biological activity, contain starch granules, and are surrounded by a mucilage layer. Root treatments using different concentrations of 4-HBA affected the development of RBCs and root growth, and induced the secretion of salicylic acid from the RBCs, which can stimulate, induce, and maintain the activity of antioxidant enzymes to some extent in the cells under 4-HBA stress. Conclusion Grapevine RBCs may resist 4-HBA stress by increasing its number and enhancing their secondary metabolism and SA accumulation, which can induce the antioxidant defense system of the cells.
机译:AIMS RHO-羟基苯甲酸(4-HBA)是导致葡萄植物的主要诱导毒素之一。根边界细胞(RBCS)在植物防御中发挥着重要作用。虽然在许多植物中已经调查了RBC,但葡萄藤中的相关信息有限。在这项研究中,我们旨在探讨葡萄(血管肝肺炎X血管杆菌菌布)'β'中RBC的发育特性及其对4-HBA应激的反应。方法在激光扫描共聚焦显微镜下观察到RBC。通过高效液相色谱法测定RBCS粘液的酚酸组合物。通过定量的实时聚合酶链反应测定基因表达水平。结果Grapevine RBCs具有高生物活性,含有淀粉颗粒,并被粘液层包围。使用不同浓度的4-HBA的根治疗影响RBC和根系生长的发展,并诱导来自RBC的水杨酸的分泌,这可以在4岁以下的细胞中刺激,诱导和维持抗氧化酶的活性。 -HBA压力。结论通过增加其数量并增强它们的次级代谢和SA积累,可以通过增加抗抵抗4-HBA应力,并占据细胞的抗氧化防御系统来抵抗4-HBA应力。

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