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首页> 外文期刊>Plant and Soil >Inoculation with five diazotrophs alters nitrogen metabolism during the initial growth of sugarcane varieties with contrasting responses to added nitrogen
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Inoculation with five diazotrophs alters nitrogen metabolism during the initial growth of sugarcane varieties with contrasting responses to added nitrogen

机译：与五种重氮化接种在甘蔗品种的初始生长期间改变氮代谢，对比加入氮气的反应

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Aims We examined the influence of inoculation with five species/strains of diazotrophic bacteria on the modulation of two enzymes involved in the assimilation of N and on the soluble N fractions in the sugarcane varieties RB867515 (adapted for low fertility soils) and IACSP95-5000 (adapted for medium to high fertility soils) under high- (3 mM) and low (0.3 mM)-N conditions in hydroponic cultivation for 59 days. Methods The sugarcane plants were produced in three steps to obtain the hydroponic cultivation: the supply of 3 mM N for 30 days (first harvest), N depletion for 72 h (second harvest), and cultivation in high- and low-N conditions over 26 days (final harvest). Inoculation was performed by immersion of the minisetts in a diluted solution of five diazotrophic bacteria. After the final harvest, plants were divided into roots and shoots to assess their dry weight and N, P, and K accumulation. Results The variety played an important role in the interaction with diazotrophs, each showing distinct behavior in the activity of their N-assimilation enzymes. The nitrate reductase activity (NRa-EC 1.7.1.1) was increased in var. RB867515 by 26% in the shoots and by 48% in the roots after 72 h under N depletion, while var. IACSP95-5000 showed a reduced enzymatic activity in the roots (by 62%) but not in the shoots. Under high-N conditions, the inoculated IACSP95-5000 plants showed 31% higher glutamine synthetase activity (GSa-EC 6.31.2) compared with 19% in RB867515. Under low-N conditions, the GSas were 21% and 16% higher in the inoculated RB867515 and IACSP95-5000 plants, respectively, compared with that of the control. The content of nitrogen in the form of nitrate (N-nitrate) confirmed these varietal differences, but the soluble sugar content did not. Conclusions The varieties utilized N sources differently, and inoculation modified the activity of two N-assimilation enzymes as well as the biomass accumulation, with the highest improvement seen in the low fertility-adapted variety RB867515; it showed a greater response to inoculation compared with that of the high fertility-adapted variety IACSP95-5000, with an increase in biomass and nutrient accumulation (N, P, K), especially when cultivated under low-N conditions. This suggests that the best response to inoculation with diazotrophs will be achieved using low fertility-adapted sugarcane varieties under low-N conditions.
机译：目的我们研究了接种与五种重氮营养细菌的影响对甘蔗品种RB867515中甘蔗品种中可溶性的两种酶的调节（适用于低生育土壤）和IACSP95-5000（适用于高（3mm）和低（0.3毫米）-N的水培培养条件下的中高生育土壤）59天。方法采用三个步骤生产甘蔗植物，得到水培培养：30天（第一次收获）的供应量为72小时（第二收获），并在高度和低N条件下培养26天（最终收获）。通过浸入稀释的五种虚拟营养细菌的稀释溶液中进行接种。在最终收获后，植物分为根部并射击，以评估其干重和N，P和K积累。结果多样性在与二氮化术相互作用中发挥着重要作用，每种在其N-同化酶的活性中显示出不同的行为。硝酸还原酶活性（NRA-EC 1.7.1.1）在VAR中增加。在N耗尽下，射击后RB867515射击射击26％，在72小时后，在根系中的48％。 IACSP95-5000在根中表现出降低的酶活性（62％）但不在芽中。在高氮条件下，接种的IACSP95-5000植物显示出31％的谷氨酰胺合成酶活性（GSA-EC 6.31.2），而RB867515中的19％相比。在低N条件下，与对照相比，GSA分别在接种的RB867515和IACSP95-5000植物中分别为21％和16％。硝酸盐形式（N-硝酸盐）形式的氮的含量证实了这些变异差异，但可溶性糖含量没有。结论各种不同的态源不同，并且接种改性了两种同化酶的活性以及生物质积累，在低生育适应的品种RB867515中看到的最高改善;它表明与高生物适应品种IACSP95-5000相比的接种反应，尤其是在低N条件下培养时的生物质和营养积累（N，P，K）增加。这表明在低N条件下使用低生育适应的甘蔗品种将实现对具有二位管的接种的最佳反应。

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