...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant and Soil >Characterization of the main magnesium transporters mediating different Mg translocation from root to shoot between Mg-tolerant and Mg-sensitive Brassica napus cultivars under magnesium deficiency stress
【24h】

Characterization of the main magnesium transporters mediating different Mg translocation from root to shoot between Mg-tolerant and Mg-sensitive Brassica napus cultivars under magnesium deficiency stress

机译：在缺乏缺陷胁迫下，从根系中介导不同Mg易位的主要Mg易位的主要镁转运蛋白的特征

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background and aims Magnesium (Mg) deficiency impacts many metabolic processes in Brassica napus (B. napus), leading to yield loss. However, the mechanism of Mg2+ uptake and translocation in B. napus remains unknown. Methods After screening 39 genotypes of B. napus under Mg-deficient conditions, the cultivars P160 and P153 were selected according to their Mg transfer factor (TF) from root to shoot. We further characterized these two genotypes under Mg-deficiency by analyzing chlorophyll concentration, malondialdehyde and peroxidase activity, and reducing sugar concentration in leaves. Additionally, we performed transcriptomics and qRT-PCR assays on P153 and P160 shoots and roots. The identified functional genes involved in Mg transport were characterized by functional assays in yeast and Arabidopsis mutants. Results The physiological analysis revealed that P160 (Mg tolerance cultivar; Mg-T) is more tolerant than P153 (Mg sensitive cultivar; Mg-S) under magnesium-deficient environments. Transcriptomics and qRT-PCR assays revealed that transcript levels of BnMGT1-2 and BnMGT6-1 were more significantly up-regulated in the shoot of Mg-T cultivar than that of the Mg-S cultivar under Mg limitation. Functional assays of BnMGT1-2 and BnMGT6-1 reveals that BnMGT1-2 and BnMGT6-1 are the two main functional Mg transporters mediating Mg translocation from root to shoot under low Mg conditions. Conclusion Mg-T is more efficient in the translocation of Mg from root to shoot than Mg-S. BnMGT1-2 and BnMGT6-1 should be the two main Mg transporters associated with Mg translocation under Mg deficiency condition, which caused the different Mg efficiency between the Mg-T and Mg-S.
机译：背景和AIMS镁（Mg）缺乏影响芸苔（B. Napus）的许多代谢过程，导致产量损失。然而，B. Napus中Mg2 +摄取和易位的机制仍然未知。方法在缺乏缺陷条件下筛选B. Napus的39个基因型后，根据其Mg转移因子（TF）从根部拍摄的Mg转移因子（TF）选择。通过分析叶绿素浓度，丙二醛和过氧化物酶活性，并降低叶片中的糖浓度，我们进一步表现了这两种基因型。另外，我们在P153和P160芽和根上进行了转录组和QRT-PCR测定。酵母和拟南芥突变体中的功能测定表征涉及Mg传输的鉴定的官能基因。结果生理分析显示，在缺镁环境下，P160（Mg耐受品种; Mg-T）比P153（Mg敏感品种; MG-S）更耐受。转录组织和QRT-PCR测定显示，在Mg-T品种的芽中比MG-S品种的拍摄更显着上调，BNMGT1-2和BNMGT6-1的转录物水平比MG限制在MG-S栽培品种中更显着上调。 BNMGT1-2和BNMGT6-1的功能测定揭示了BNMGT1-2和BNMGT6-1是介导从低Mg条件下从根部拍摄Mg易位的两个主要功能Mg转运蛋白。结论MG-T在从根部转移到射击比MG-S的易位更有效。 BNMGT1-2和BNMGT6-1应该是与Mg缺乏条件下的Mg易位相关的两个主要的Mg转运蛋白，这导致MG-T和MG-S之间的不同MG效率。

著录项

  • 来源
    《Plant and Soil》 |2019年第2期|共16页
  • 作者

    Sun Juanjuan; Li Shengting; Wang Shufeng; Xiao Zhongchun; Zhang Zhen; Li Jiana; Luo Feng; Li Nannan;

  • 作者单位

    Southwest Univ Coll Resources &

    Environm Chongqing Key Lab Bioresource Energy Chongqing 400715 Peoples R China;

    Southwest Univ Coll Resources &

    Environm Chongqing Key Lab Bioresource Energy Chongqing 400715 Peoples R China;

    Southwest Univ Coll Resources &

    Environm Chongqing Key Lab Bioresource Energy Chongqing 400715 Peoples R China;

    Southwest Univ Acad Agr Sci Chongqing 400715 Peoples R China;

    Southwest Univ Coll Resources &

    Environm Chongqing Key Lab Bioresource Energy Chongqing 400715 Peoples R China;

    Southwest Univ Acad Agr Sci Chongqing 400715 Peoples R China;

    Southwest Univ Coll Resources &

    Environm Chongqing Key Lab Bioresource Energy Chongqing 400715 Peoples R China;

    Southwest Univ Coll Resources &

    Environm Chongqing Key Lab Bioresource Energy Chongqing 400715 Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 土壤学;
  • 关键词

    Brassica napus; Mg deficiency; Transcriptomic analysis; Mg transporter; Genotypes; MGT family;

    机译：Brassica Napus;mg缺乏;转录组分析;Mg运输扣;基因型;MGT家族;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号