Identification and molecular mapping of        <i >indicaindica        high‐tillering dwarf mutant        <i >htd4htd4        , a mild phenotype allelic mutant of        <i >D14D14        in rice (        <i >Oryza sativaOryza sativa        L.)

Wang Y.‐P.; Tang S.‐Q.; Chen H.‐Z.; Wu Z.‐F.; Zhang H.; Duan E.‐C.; Shi Q.‐H.; Wu Z.‐M.; Fransz P.

摘要

Abstract > <list formatted="paragraph" style="bulleted" xml:id="plb12612-list-0001"> <listItem> Metabolism of strigolactones ( <fc>SL</fc> s) can improve the efficiency of nutrient use by regulating the development of roots and shoots in crops, making them an important research focus for molecular breeding. However, as a very important plant hormone, the molecular mechanism of <fc>SL</fc> signal transduction still remains largely unknown. </listItem> <listItem> In this study, we isolated an indica high‐tillering dwarf mutant 4 ( htd4 ), a spontaneous mutant of rice, from the restorer line Gui99. </listItem> <listItem> Mapping and sequencing analysis showed that htd4 was a novel allelic mutant of D14 , in which a single base substitution forms a premature termination codon. Quantitative <fc>RT</fc> ‐ <fc>PCR</fc> analyses revealed that expression levels of the genes D10 , D17 , D27 , D3 and D14 increased significantly, while expression of D53 decreased in htd4 , compared with the wild type. A subcellular localisation assay showed that the mutant of D14 in htd4 did not disturb the normal localisation of D14 proteins. However, a Bi <fc>FC</fc> assay suggested that the mutant‐type D14 could not interact with D3. Additionally, compared with other </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“main”XML：ID =“PLB12612-Abs-Abs-0001”> <标题类型=“main”>抽象</ title> > <list格式=“段落”样式=“项目符号”XML：ID =“plb12612-list-0001”> <listitem>滴答物的代谢（<fc> sl </ fc> s）可以通过调节作物中的根源和芽的开发来提高营养利用的效率，使其成为重要的研究重点用于分子育种。然而，作为一个非常重要的植物激素，<Fc> SL </ Fc>信号转导的分子机制仍然很大程度上是未知的。本研究中的</ listitem> <listitem>，我们分离出一个 indica </ i>高分蘖矮小突变体4（ htd4 </ i>），从恢复系Gui99 。 </ listitem> <listitem>映射和测序分析表明， HTD4 </ i>是 D14的新等位基因突变体，其中单个基本取代形成过早终止密码子。定量<FC> RT / Fc> - <Fc> PCR </ Fc>分析显示，基因的表达水平 D10 </ i>， D17 </ i>， D27 </ I>， D3 </ i>和 D14 </ i>显着增加，而 D53的表达在 HTD4 </ i>中减少，与野生型相比。亚细胞定位测定结果显示D14中的D14突变体在 HTD4 </ i>下没有干扰D14蛋白的正常定位。然而，Bi <Fc> Fc </ Fc>测定表明突变体D14不能与D3相互作用。此外，与其他相比 </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-29390/'>《Plant biology》</a> <b style="margin: 0 2px;">|</b><span>2017年第6期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wang Y.‐P.&option=202" target="_blank" rel="nofollow">Wang Y.‐P.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Tang S.‐Q.&option=202" target="_blank" rel="nofollow">Tang S.‐Q.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Chen H.‐Z.&option=202" target="_blank" rel="nofollow">Chen H.‐Z.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wu Z.‐F.&option=202" target="_blank" rel="nofollow">Wu Z.‐F.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Zhang H.&option=202" target="_blank" rel="nofollow">Zhang H.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Duan E.‐C.&option=202" target="_blank" rel="nofollow">Duan E.‐C.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Shi Q.‐H.&option=202" target="_blank" rel="nofollow">Shi Q.‐H.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Wu Z.‐M.&option=202" target="_blank" rel="nofollow">Wu Z.‐M.;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Fransz P.&option=202" target="_blank" rel="nofollow">Fransz P.;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>Key Laboratory of Crop Physiology Ecology and Genetic Breeding of Jiangxi ProvinceJiangxi Agricultural UniversityNanchang China;</p> <p>Key Laboratory of Crop Physiology Ecology and Genetic Breeding of Jiangxi ProvinceJiangxi Agricultural UniversityNanchang China;</p> <p>Key Laboratory of Crop Physiology Ecology and Genetic Breeding of Jiangxi ProvinceJiangxi Agricultural UniversityNanchang China;</p> <p>Key Laboratory of Crop Physiology Ecology and Genetic Breeding of Jiangxi ProvinceJiangxi Agricultural UniversityNanchang China;</p> <p>National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural SciencesBeijing China;</p> <p>National Key Facility for Crop Gene Resources and Genetic ImprovementChinese Academy of Agricultural SciencesBeijing China;</p> <p>Key Laboratory of Crop Physiology Ecology and Genetic Breeding of Jiangxi ProvinceJiangxi Agricultural UniversityNanchang China;</p> <p>Key Laboratory of Crop Physiology Ecology and Genetic Breeding of Jiangxi ProvinceJiangxi Agricultural UniversityNanchang China;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/184.html" title="植物学">植物学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=D14&option=203" rel="nofollow">D14;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=dwarf&option=203" rel="nofollow">dwarf;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=fine mapping&option=203" rel="nofollow">fine mapping;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=high tillering&option=203" rel="nofollow">high tillering;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Oryza sativa&option=203" rel="nofollow">Oryza sativa;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=strigolactones ( SL s)&option=203" rel="nofollow">strigolactones ( SL s);</a> </p> <div class="translation"> 机译：D14;矮人;精细映射;高分蘖;oryza sativa;strigolactones（sl s）; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" 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tuijian_authcolor">,薛庆中</a> <span> <a href="/journal-cn-56831/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 生物技术 </a> </span> <span> . 2004</span><span>,第6期</span> </span> </div> </li> <li> <div> <b>4. </b><a class="enjiyixqcontent" href="/academic-journal-cn_detail_thesis/0201292372799.html">上位性和QTL×环境互作对水稻(Oryza sativa L.)抽穗期的影响(英文)</a> <b>[J]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘桂富&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘桂富</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=杨剑&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,杨剑</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=徐海明&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,徐海明</a> <span> <a href="/journal-cn-57195/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 遗传学报：英文版 </a> </span> <span> . 2007</span><span>,第7期</span> </span> </div> </li> <li> <div> <b>5. </b><a class="enjiyixqcontent" href="/academic-conference-cn_meeting-14868_thesis/020221258163.html">杂交水稻(Oryza sativa L.)种子固有细菌群落多样性探究</a> <b>[C]</b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘琳&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 刘琳</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=刘洋&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,刘洋</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=宋未&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor">,宋未</a> <span> <a href="/conference-cn-14868/" target="_blank" rel="nofollow" class="tuijian_authcolor"> . 2009年中国微生物生态学年会 </a> <span> <span> . 2009</span> </span> </div> </li> <li> <div> <b>6. </b><a class="enjiyixqcontent" href="/academic-degree-domestic_mphd_thesis/020311561923.html">水稻(Oryza sativa L.)光呼吸突变体线粒体丝氨酸羟甲基转移酶基因克隆及功能分析</a> <b>[A] </b> <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=郭玉朋&option=202" target="_blank" rel="nofollow" class="tuijian_auth tuijian_authcolor"> . 郭玉朋</a> <span> . 2010</span> </span> </div> </li> </ul> <ul style="display: none;"> <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/patent-detail/06130401314043.html">method for reducing plant height, method for identifying a br1 allele or a dw5 allele in a plant population, semianan plant, semianan sorghum plant, recombinant DNA construction, plant cell, plant rna sequence, seed, plant modified semiannum sorghum, dwarf sorghum plant, mutant maize plant, method to reduce plant height, sorghum plant, sorghum plant population, method to reduce the frequency of reversion of a dwarf 3 (dw3) allele in a plant sorghum in a high phenotype, rice plant, marker assisted selection method of a plant</a> <b>[P]</b> . <span> 外国专利：  BR112020001844A2 </span> <span> . 2020-10-06</span> </div> <p class="zwjiyix translation" style="max-width: initial;height: auto;word-break: break-all;white-space: initial;text-overflow: initial;overflow: initial;"> <span>机译：降低植物高度的方法，在植物种群中鉴定br1等位基因或dw5等位基因的方法，Semianan植物，Semianan高粱植物，重组DNA构建，植物细胞，植物rna序列，种子，植物修饰的Semiannum高粱，矮化高粱植物，玉米突变体植物，降低植物高度的方法，高粱植物，高粱植物种群，降低高表型植物高粱中矮3（dw3）等位基因回复频率的方法，水稻，标记辅助选择方法厂 </span> </p> </li> </ul> </div> </div> </div> <div class="theme cardcommon" style="overflow: auto;display:none"> <h3 class="all_title" id="enpatent55">相关主题</h3> <ul id="subject"> </ul> </div> </div> </div> </div> <div class="right rightcon"> <div class="details_img cardcommon clearfix" style="margin-bottom: 10px;display:none;" > </div> </div> </div> <div id="thesis_get_original1" class="downloadBth" style="bottom: 19px;z-index: 999;" onclick="ywcd('0704024503669','4',7,2,1,'',this,24)" class="delivery" prompt="010401" title="通过人工服务将文献原文发送至邮箱" >获取原文</div> <div class="journalsub-pop-up" style="display: none"> <div class="journal-sub"> 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Identification and molecular mapping of indicaindica high‐tillering dwarf mutant htd4htd4 , a mild phenotype allelic mutant of D14D14 in rice ( Oryza sativaOryza sativa L.)

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