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首页> 外文期刊>Plant Cell Reports >High expression of GUS activities in sweet potato storage roots by sucrose-inducible minimal promoter
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High expression of GUS activities in sweet potato storage roots by sucrose-inducible minimal promoter

机译：通过蔗糖诱导的最小促进剂在甘薯储存根中GUS活动的高表达

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Key message We developed transgenic sweet potato with Spo(min) (sucrose-inducible minimal promoter)-GUS gene-fused constructs. Induced GUS activities by Spo(min) were higher than those by CaMV 35S promoter. We developed transgenic sweet potato (Ipomoea batatas L. Lam. cv. Kokei no. 14) plants with Spo(min) (sucrose-inducible minimal promoter)-GUS gene-fused constructs with signal peptides for sorting to cytosol, apoplast and ER, and we analyzed the GUS expression pattern of cut tissue after sucrose treatment. Induced GUS activities by Spo(min) were several hundred times higher than those by the CaMV 35S promoter. Also, GUS activities in storage roots induced with a Spo(min)-cytosol-GUS construct were higher than those with either Spo(min)-apoplast or -ER-GUS constructs. The induced GUS activities by Spo(min) were higher in storage roots without sucrose treatment than those with sucrose treatment. Chilling (4 degrees C) storage roots with Spo(min) constructs for 4 weeks produced higher GUS activities than in storage roots stored at 25 degrees C for 4 weeks. The calculated maximum GUS content in the storage roots was up to about 224.2 mu g/g fresh weight. The chilling treatment increased the free sucrose content in the storage roots, and this increase in endogenous sugar levels induced increased GUS activities in the storage roots. Therefore, Spo(min) appears to be a useful promoter to develop protein production systems using sweet potato variety Kokei no. 14 storage roots by postharvest treatment.
机译：关键消息我们开发了转基因甘薯的型孢子（分钟）（蔗糖可诱导的最小启动子） - 基因融合构建体。 Spo（min）诱导的GUS活动高于CAMV 35s启动子的GUS活动。我们开发了转基因甘薯（Ipomoea Batatas L. Lam。CV。Kokei No.14）孢子（分钟）（蔗糖可诱导的最小启动子）植物 - 基因融合构建与信号肽，用于分选于细胞溶质，胰糖和呃，我们分析了蔗糖处理后切割组织的GUS表达模式。 Spo（min）诱导的GUS活动比CAMV 35s启动子高的数百倍。此外，用SPO（min）-cytosol-GUS构建体诱导的储存根部的GUS活性高于孢子（min）-Apoplast或-er-Gus构建体的储存根部。通过蔗糖处理的储存根（min）诱导的GUS活动比蔗糖处理的储存较高。冷却（4摄氏度）储存根与烟型（分钟）构建体4周产生较高的GUS活动，而不是在25摄氏度储存4周的储存根部。存储根中的计算最大GUS含量高达约224.2μmg/ g鲜重。冷却治疗增加了储存根部的游离蔗糖含量，内源性糖水平的这种增加诱导了储存根部的GUS活动增加。因此，Spo（min）似乎是使用甘薯品种Kokei NO产生蛋白质生产系统的有用启动子。通过采后处理的14个存储根。

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