A single amino acid change at position 96 (Arg to His) of the sweetpotato Orange protein leads to carotenoid overaccumulation

Kim So-Eun; Kim Ho Soo; Wang Zhi; Ke Qingbo; Lee Chan-Ju; Park Sul-U; Lim Ye-Hoon; Park Woo Sung; Ahn Mi-Jeong; Kwak Sang-Soo

首页> 外文期刊>Plant Cell Reports >A single amino acid change at position 96 (Arg to His) of the sweetpotato Orange protein leads to carotenoid overaccumulation

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A single amino acid change at position 96 (Arg to His) of the sweetpotato Orange protein leads to carotenoid overaccumulation

机译：在甘薯橙蛋白的96（arg到他）的单一氨基酸变化导致胡萝卜素过度累计

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Key message IbOr-R96H resulted in carotenoid overaccumulation and enhanced abiotic stress tolerance in transgenic sweetpotato calli. The Orange (Or) protein is involved in the regulation of carotenoid accumulation and tolerance to various environmental stresses. Sweetpotato IbOr, with strong holdase chaperone activity, protects a key enzyme, phytoene synthase (PSY), in the carotenoid biosynthetic pathway and stabilizes a photosynthetic component, oxygen-evolving enhancer protein 2-1 (PsbP), under heat and oxidative stresses in plants. Previous studies of various plant species demonstrated that a single-nucleotide polymorphism (SNP) from Arg to His in Or protein promote a high level of carotenoid accumulation. Here, we showed that the substitution of a single amino acid at position 96 (Arg to His) of wild-type IbOr (referred to as IbOr-R96H) dramatically increases carotenoid accumulation. Sweetpotato calli overexpressing IbOr-WT or IbOr-Ins exhibited 1.8- or 4.3-fold higher carotenoid contents than those of the white-fleshed sweetpotato Yulmi (Ym) calli, and IbOr-R96H overexpression substantially increased carotenoid accumulation by up to 23-fold in sweetpotato calli. In particular, IbOr-R96H transgenic calli contained 88.4-fold higher levels of beta-carotene than those in Ym calli. Expression levels of carotenogenesis-related genes were significantly increased in IbOr-R96H transgenic calli. Interestingly, transgenic calli overexpressing IbOr-R96H showed increased tolerance to salt and heat stresses, with similar levels of malondialdehyde to those in calli expressing IbOr-WT or IbOr-Ins. These results suggested that IbOr-R96H is a useful target for the generation of efficient industrial plants, including sweetpotato, to cope with growing food demand and climate change by enabling sustainable agriculture on marginal lands.

机译：关键消息Ibor-R96导致转基因甘薯愈伤组织中的类胡萝卜素过度累积和增强的非生物胁迫耐受性。橙色（或）蛋白参与调节类胡萝卜素积累和对各种环境应激的耐受性。具有强大的寄生酶活性，在类胡萝卜素生物合成途径中保护关键酶，植物植物合酶（PSY），稳定在植物中的热和氧化胁迫下稳定光合作用组分，氧化增强剂蛋白2-1（PSBP）的关键酶。以前对各种植物物种的研究表明，从arg到他的In或蛋白质的单核苷酸多态性（SNP）促进了高水平的类胡萝卜素积累。在这里，我们表明，在野生型IBOR（称为IBOR-R96H）的位置96（ARG到他）的单个氨基酸的取代显着增加了类胡萝卜素积累。 SweetPoTato Calli过表达Ibor-Wt或Ibor-ins的表现出比白皮甜点Yulmi（YM）Calli（YM）愈伤组织的含量为1.8或4.3倍，并且Ibor-R96h过表达的表达式大幅增加了Carotenoid积累了23倍SweetPotato Calli。特别是，Ibor-R96h转基因Calli含有88.4倍的β-胡萝卜素，而不是YM Calli的β-胡萝卜素。 IBor-R96H转基因愈伤组织中，雌雌生成相关基因的表达水平显着增加。有趣的是，转基因Calli过表达Ibor-R96h对盐和热应激的耐受性增加，具有与表达Ibor-wt或铁合油的疾病中的丙二醛相似。这些结果表明，IBOR-R96H是通过在边缘土地上实现可持续农业的可持续农业来应对含有甘薯的有效工厂，包括甘薯的高效工业厂，以应对粮食需求和气候变化的有用目标。

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来源
《Plant Cell Reports》 |2019年第11期|共10页
作者
Kim So-Eun; Kim Ho Soo; Wang Zhi; Ke Qingbo; Lee Chan-Ju; Park Sul-U; Lim Ye-Hoon; Park Woo Sung; Ahn Mi-Jeong; Kwak Sang-Soo;
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作者单位

KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

Northwest A&

F Univ Inst Soil &

Water Conservat Yangling 712100 Shaanxi Peoples R China;

Northwest A&

F Univ Inst Soil &

Water Conservat Yangling 712100 Shaanxi Peoples R China;

KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

Gyeongsang Natl Univ Coll Pharm 501 Jinjudae Ro Jinju 52828 South Korea;

Gyeongsang Natl Univ Coll Pharm 501 Jinjudae Ro Jinju 52828 South Korea;

KRIBB Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

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原文格式 PDF
正文语种 eng
中图分类植物细胞学;
关键词
Carotenoid; IbOr; IbOr-R96H; Metabolic engineering; Sweetpotato;

机译：类胡萝卜素;ibor;ibor-r96h;代谢工程;甜点;

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专利

1. A single amino acid change at position 96 (Arg to His) of the sweetpotato Orange protein leads to carotenoid overaccumulation [J] . Kim So-Eun, Kim Ho Soo, Wang Zhi, Plant Cell Reports . 2019,第11期

机译：在甘薯橙蛋白的96（arg到他）的单一氨基酸变化导致胡萝卜素过度累计
2. Site-specific single amino acid changes to Lys or Arg in the centralregion of the movement protein of a hybrid bromovirus are required foradaptation to a nonhost [J] . Sasaki N, Fujita Y, Mise K, Virology . 2001,第1期

机译：为了适应非宿主，需要在混合型溴病毒的运动蛋白中心区域对Lys或Arg进行位点特异性单氨基酸改变
3. The exchange of one single amino acid at position 71 of the DR4 beta-chain leads to significant differences in antigen processing and presentation of a human autoantigen chaperoned by a member of the HSP70 family [J] . S Roth, N Willcox, MP Mayer, Arthritis Research . 2001,第SupplaA期

机译：DR4β链第71位的单个氨基酸交换导致抗原加工和HSP70家族成员陪伴的人自身抗原伴侣蛋白呈递的显着差异
4. Changes in tissue amino acid composition and protein metabolism in piglets due to a limiting supply of total sulphur amino acids [C] . J.A. Conde-Aguilera, C. Cobo-Ortega, N. LeFloch, International Symposium on Energy and Protein Metabolism and Nutrition . 2013

机译：由于硫磺氨基酸的限制供应，仔猪组织氨基酸组成和蛋白质代谢的变化
5. Use of the neonatal piglet as a model for protein and amino acid metabolism in premature infants: 1. Use of body carbohydrate, lipid and protein during total and partial starvation. 2. Negative impact of dexamethasone treatment on growth, protein synthesis and amino acid oxidation. [D] . Mickelson, Barbara Dolores. 2001

机译：将新生仔猪用作早产婴儿蛋白质和氨基酸代谢的模型：1.在全部和部分饥饿期间使用体内碳水化合物，脂质和蛋白质。 2.地塞米松处理对生长，蛋白质合成和氨基酸氧化的负面影响。
6. A Single Amino Acid Substitution in an ORANGE Protein Promotes Carotenoid Overaccumulation in Arabidopsis [O] . Hui Yuan, Katherine Owsiany, T.E. Sheeja, 2015

机译：ORANGE蛋白中的单个氨基酸取代促进拟南芥中类胡萝卜素的过度积累。
7. A Single Amino Acid Substitution in an ORANGE Protein Promotes Carotenoid Overaccumulation in Arabidopsis [O] . Hui Yuan, Katherine Owsiany, T.E. Sheeja, 2015

机译：橙色蛋白质中的单一氨基酸取代促进了拟南芥的类胡萝卜素过度累积

A single amino acid change at position 96 (Arg to His) of the sweetpotato Orange protein leads to carotenoid overaccumulation

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