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首页> 外文期刊>Plant Cell Reports >Differential anthocyanin accumulation in radish taproot: importance of RsMYB1 gene structure
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Differential anthocyanin accumulation in radish taproot: importance of RsMYB1 gene structure

机译：萝卜根塔的差异血清粘蛋白积累：RSMYB1基因结构的重要性

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摘要

Key message RsMYB1a was the crucial MYB, and RsbHLH4 is the essential partner in regulating the anthocyanin biosynthesis in radish. There are four color types of radish according to whether or not the anthocyanin accumulates in the skin and flesh of taproot. Red radishes accumulate a substantial amount of anthocyanins in both the skin and flesh. It is well known that the MYB-bHLH-WD40 transcription factor(s) complex regulates the biosynthesis of anthocyanin in plants. Here in, four candidate MYB and bHLH genes, RsMYB1a, RsMYB1b, RsbHLH2 and RsbHLH4, were isolated from red radish 'Hongxin 1'. The expression of RsbHLH4 and the two structural genes RsANS and RsUFGT was significantly positively correlated with anthocyanin contents. The expression of RsMYB1a was also highly correlated with anthocyanin accumulation, particularly when the white flesh sample of 'Hongxin 1-1' was excluded. The transient expression of RsMYB1a in the radish cotyledon and leaf induced anthocyanin accumulation with even stronger promoting role when expression in combination with RsbHLH4. These results suggested that RsMYB1a was the crucial MYB, and that RsbHLH4 is an essential partner in regulating the biosynthesis of anthocyanins in radish. The low or undetectable RsbHLH4 expression paralleled the lack of anthocyanin accumulation in the white flesh of 'Hongxin 1-1' and 'Shaguan 1'. Assays demonstrated that RsMYB1a interacted with RsbHLH4 and activated the expression of RsbHLH4. Notably, all the dark red radish cultivars have a longer RsMYB1a genomic DNA sequence, while the short and nonfunctional RsMYB1a is present in non-red cultivars. The length of the first intron and the presence of an early stop codon of RsMYB1 might underlie the differential anthocyanin accumulation in the radish taproot.
机译：关键消息RSMYB1A是关键MYB，RSBHLH4是调节萝卜中的花青素生物合成的基本伴侣。根据花青素是否积聚在皮根皮和肉体中，有四种颜色类型的萝卜。红色萝卜在皮肤和肉体中积累了大量的花青素。众所周知，MYB-BHLH-WD40转录因子复合体调节植物中花青素的生物合成。在这里，四个候选MYB和BHLH基因，RSMYB1A，RSMYB1B，RSBHLH2和RSBHLH4，从红萝卜“红光1”中分离出来。 RSBHLH4的表达和两个结构基因RSANS和RSUFGT与花青素含量显着呈正相关。 RSMyB1a的表达也与花青素积累的高度相关，特别是当排除“红鑫1-1”的白肉样品时。 rsmyb1a在萝卜子叶和叶片中的瞬时表达，诱导花青素积累，甚至更强的促进作用，当表达与rsbhlh4组合时。这些结果表明RSMyB1A是关键的MYB，RSBHLH4是调节萝卜中花青素生物合成的重要伙伴。低或无法检测到的RSBHLH4表达并联在“红鑫1-1”和“Shaguan 1”的白色肉中缺乏缺乏花青素积累。测定证明RSMyB1A与RSBHLH4相互作用并激活RSBHLH4的表达。值得注意的是，所有深红色的萝卜品种都有更长的RSMyB1A基因组DNA序列，而短官能的RSMyB1A存在于非红细胞中。第一个内含子的长度和RSMyB1的早期止核密码子的存在可能会使萝卜根根簇的差异化花青素积累提高。

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  • 来源
    《Plant Cell Reports》 |2020年第2期|共10页
  • 作者

    Lai Biao; Cheng Yuanyi; Liu Hong; Wang Qiuxia; Wang Qi; Wang Chunlan; Su Rui; Chen Fabo; Wang Huicong; Du Lina;

  • 作者单位

    Yangtze Normal Univ Sch Adv Agr &

    Bioengn Chongqing 408100 Peoples R China;

    Yangtze Normal Univ Sch Adv Agr &

    Bioengn Chongqing 408100 Peoples R China;

    Yangtze Normal Univ Sch Adv Agr &

    Bioengn Chongqing 408100 Peoples R China;

    Yangtze Normal Univ Sch Adv Agr &

    Bioengn Chongqing 408100 Peoples R China;

    Yangtze Normal Univ Sch Adv Agr &

    Bioengn Chongqing 408100 Peoples R China;

    Yangtze Normal Univ Sch Adv Agr &

    Bioengn Chongqing 408100 Peoples R China;

    Yangtze Normal Univ Sch Adv Agr &

    Bioengn Chongqing 408100 Peoples R China;

    Yangtze Normal Univ Sch Adv Agr &

    Bioengn Chongqing 408100 Peoples R China;

    Yangtze Normal Univ Sch Adv Agr &

    Bioengn Chongqing 408100 Peoples R China;

    Yangtze Normal Univ Sch Adv Agr &

    Bioengn Chongqing 408100 Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物细胞学;
  • 关键词

    Red radish; Anthocyanins; MYB; bHLH; Transcriptional regulation;

    机译：红萝卜;花青素;myb;bhlh;转录规则;

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