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首页> 外文期刊>Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants >Photoperiod modulates growth, morphoanatomy, and linalool content in Lippia alba L. (Verbenaceae) cultured in vitro
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Photoperiod modulates growth, morphoanatomy, and linalool content in Lippia alba L. (Verbenaceae) cultured in vitro

机译:光周期调节Lippia Alba L.(vallenaceae)在体外培养的生长,morphoanatomy和linalool含量

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Interactions between circadian clock regulation and metabolic responses are believed to explain the importance of rhythmic behavior in plant growth and survival. Lippia alba is an important species because of the medicinal properties of its essential oil extract. The objective of this work was to evaluate the effect of photoperiod on anatomy, growth, essential oil profile, and the expression of genes related to the synthesis of monoterpenes, sesquiterpenes, and the circadian clock in L. alba grown in vitro. The plants were cultured in vitro under different photoperiods (4, 8, 16, and 24 h of light) and irradiance of 41 mu mol m(-2) s(-1). After 40 days of culture, results showed that L. alba presented high physiological plasticity under different photoperiods, with improved performance when exposed to continuous light. The best growth; anatomical organization of the mesophyll, stem, roots, and bundles; amount of photosynthetic pigments; photosynthetic rate; and protein synthesis occurred under a photoperiod of 24 h. The biosynthesis of linalool, the major compound, was increased under the 24-h photoperiod, possibly due to reduced geraniol synthesis. These findings allow a better understanding of how photoperiod acts in the regulation of primary and secondary metabolism, and especially with regard to the composition of essential oils.
机译:据信昼夜时钟调节与代谢反应之间的相互作用据信解释了植物生长和生存中节律行为的重要性。 Lippia Alba是一种重要的物种,因为其精油提取物的药用特性。这项工作的目的是评估光周期对解剖学,生长,精油概况的影响,以及与在体外L. Alba在体外生长的单波培,Sesquiterpenes和昼夜点昼夜的基因的表达。在不同的光周期(4,8,16和24小时的光)下在体外培养植物,并辐照为41μmm(-2)s(-1)。在培养40天后,结果表明,L. Alba在不同的光周期下呈现出高的生理可塑性,在暴露于连续光时具有改善的性能。最好的增长;叶蛋白,茎,根和束的解剖组织;光合色素的量;光合速率;在24小时的光周期下发生蛋白质合成。在24-H光周期下,烯丙酸的生物合成,主要化合物在24-H光周期下增加,可能是由于大竺烷的合成减少。这些发现允许更好地理解光周期如何在初级和次生新陈代谢的调节中,特别是关于精油的组成。

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