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首页> 外文期刊>Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants >Methyl jasmonate and salicylic acid elicitation increase content and yield of chlorogenic acid and its derivatives in Gardenia jasminoides cell suspension cultures
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Methyl jasmonate and salicylic acid elicitation increase content and yield of chlorogenic acid and its derivatives in Gardenia jasminoides cell suspension cultures

机译：茉莉酸甲酯和水杨酸引发的甲基茉莉酸和其衍生物在栀子茉莉细胞悬浮培养物中提高含量和产率

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摘要

Chlorogenic acid and its derivatives (CADs) are the bioactive components in Gardenia jasminoides Ellis (GJE) fruits but occur at a low level. In the current paper, four types of calluses were induced from GJE leaf explants, and light yellow-green calluses with a fine appearance and texture were selected for the development of a cell suspension culture to produce CADs. Additionally, cultured cells were elicited by salicylic acid (SA) and methyl jasmonate (MeJA) to increase the CADs yield. Seven CADs compounds were identified via HPLC-TOF-MS/MS, namely, 3-caffeoylquinic acid, 4-caffeoylquinic acid, 5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid, 3,5-dicaffeoyl-4-o-(3-hydroxyl-3-methyl)-glutaroylquinic acid, and malonyl-4,5-o-dicaffeoylquinic acid. This is the first identification of malonyl-4,5-o-dicaffeoylquinic acid from GJE. Both SA and MeJA significantly promoted the CADs yield, but MeJA was more effective than SA. Cells elicited by 200 A mu M MeJA showed the maximum total CADs content and yield, up to 20.98 +/- 1.1 mg g(-1) and 232.32 +/- 9.1 mg L-1, which were 19.51 times and 14.14 times those of the control, respectively. Furthermore, the total CADs extracted from cells elicited by 200 A mu M MeJA displayed a much higher antioxidant capacity than that of natural GJE fruits. Our research suggested that a GJE cell culture combined with MeJA elicitation has a promising application in natural CADs production.
机译：绿原酸及其衍生物（CADS）是Gardenia Jasminoides Ellis（GJE）果实中的生物活性成分，但在低水平下发生。在本文中，从GJE叶片外植体诱导了四种类型的愈伤组织，并选择具有精细外观和质地的淡黄绿色愈伤组织用于开发细胞悬浮培养物以产生CAD。另外，通过水杨酸（SA）和茉莉甲酸甲酯（MEJA）引发培养的细胞以增加CADS产率。通过HPLC-TOF-MS / MS鉴定出七种CADS化合物，即3-咖啡算法，4-咖啡算法酸，5-二咖啡算法，3,5-二咖啡烯酸，4,5-二咖啡烯酸，3,5-二咖啡酰基-4-O-（3-羟基-3-甲基） - 乙醇喹啉，和丙二酰-4,5-O-二氧化酰酸。这是来自GJE的丙二酰-4,5-O-二氧化酰酸的第一次鉴定。 SA和MEJA都显着促进了CADS产量，但MEJA比SA更有效。由200A亩M MejA引发的细胞显示最大的CAD含量和产率，高达20.98 +/- 1.1 mg g（-1）和232.32 +/- 9.1mg L-1，为19.51倍和14.14倍。控制分别。此外，从200A穆立MejA引发的细胞中提取的总CADS比天然GJE水果的抗氧化能力呈现得多。我们的研究表明，GJE细胞文化联合Meja Elicitation在天然CADS生产中具有有希望的应用。

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