...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants >Isolation of diploid and tetraploid cytotypes from mixoploids based on adventitious bud regeneration in Populus
【24h】

Isolation of diploid and tetraploid cytotypes from mixoploids based on adventitious bud regeneration in Populus

机译：基于杨树不定芽再生的混合卟啉单荚和四倍体细胞型分离

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Somatic chromosome doubling usually results in the production of mixoploids, which are difficult to use in breeding programs. In this study, we developed a method to isolate diploid and tetraploid cytotypes from induced mixoploids of Populus pseudo-simonii x P. nigra var. italica based on adventitious bud regeneration. We produced 75 mixoploids through colchicine-induced seed chromosome doubling in P. pseudo-simonii x P. nigra var. italica. The highest mixoploid induction frequency was 26.67 +/- 5.09% following treatment with 0.1% colchicine for 96 h. We then screened a medium for adventitious bud regeneration from leaf explants of multiple genotypes, which was Murashige and Skoog (MS) medium with 0.4 mg L-1 6-benzyladenine (BA) and 0.1 mg L-1 1-naphthaleneacetic acid (NAA). The maximum frequency of leaf differentiation was 96.67 +/- 3.33%, and the number of adventitious buds per explant was 6.17 +/- 1.02. When the medium was applied for adventitious bud regeneration of 12 mixoploid genotypes, 18 tetraploids and 26 diploids were isolated, of which five mixoploids generated both diploid and tetraploid cytotypes and seven mixoploids generated either diploids or tetraploids. Compared to diploids, tetraploids varied significantly in stomatal characteristics, micropropagation coefficient and rooting ability. Significant differences were also detected among genotypes at the same ploidy level, implying that heterozygosity also affected phenotypic variation during allopolyploidization. This study provides an effective method for isolation of diploid and tetraploid cytotypes from mixoploids and generation of full-sib diploid and tetraploid germplasms for polyploidy Populus breeding programs. Key message This paper reported an efficient method to isolate diploids and tetraploids from Populus mixoploids via adventitious bud regeneration. Paired analysis showed both heterozygosity and ploidy level contributed to morphological variation.
机译：体细胞染色体倍增通常导致产生混合物的产生，这难以在繁殖计划中使用。在这项研究中，我们开发了一种从致噬杉的诱导的混合物中分离二倍体和四倍体细胞型的方法，杨普拉·········瓦氏菌。基于不定芽再生的Italica。我们通过Colchicine诱导的种子染色体在P.Pseudo-Simonii X P. nigra VAR中加倍产生75个混合物。 Italica。在用0.1％血清晶淋，最高的混合物诱导频率为26.67 +/- 5.09％，含0.1％血清晶嘌呤96小时。然后，我们筛选了一种介质，从多种基因型的叶片外植体中筛选出不定芽再生，这是Murashige和Skoog（MS）培养基，其中0.4mg L-1 6-苄基腺嘌呤（BA）和0.1mg L-1 1-萘酰基乙酸（NAA） 。叶片分化的最大频率为96.67 +/- 3.33％，每次外植体的不定芽数为6.17 +/- 1.02。当培养基用于12个混合物基因型的不定芽再生时，分离了18只四倍体和26个二倍体，其中五个混合物产生二倍体和四倍体细胞型和7个混合物产生二倍体或四倍体。与二倍体相比，四倍体在气孔特征，微寄存系数和生根能力方面变化显着。在相同倍率水平的基因型中也检测到显着差异，这意味着杂合子在全多聚丙烯化过程中也会影响其表型变异。该研究提供了一种有效的方法，用于分离来自混合物和产生全脂二倍体和四倍体种质的多倍体杨树和四倍体种质的二倍体和四倍体细胞型。本文报告了一种有效的方法，可以通过不定芽再生将二倍体和四倍体分离杨树和四倍体。配对分析表明杂合性和倍率水平均导致形态变异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号