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Diversity of Neopestalotiopsis and Pestalotiopsis spp., Causal Agents of Guava Scab in Colombia

机译:Neopestalotiopsis和Pestalotiopsis SPP的多样性,巨蛋白核心的因果子,哥伦比亚

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Common guava (Psidiwn guajava L.) is a fruit tree of global economic importance. It is grown in Asia, South and Central America, and Hawaii for its exquisite aroma and flavor, and nutritional and medical properties. However, guava production is limited by guava scab, caused by fungi in the Pestalotiopsis genus. Characteristic symptoms of guava scab are corky, ovoid or round lesions on fruit surfaces. These lesions may thicken, affecting the flesh below and reducing fruit quality and commercial value. We characterized 81 isolates isolated from guava scab lesions on guava leaves and fruit in different regions of Colombia, and identified them as Pestalotiopsis and Neopestalotiopsis spp. We analyzed the morphology, pathogenicity, and genetic diversity of the isolates based on the sequences of the internal transcribed spacer, beta-tubulin, and elongation factor genes. Isolates were morphologically, pathogenically, and genetically diverse but the diversity did not correlate with geographical origin, or guava cultivar or tissue from which the isolates were recovered. Selected monosporic isolates included in the multiple-gene analysis were identified as belonging to two genera: Neopestalotiopsis (65 isolates with versicolorous conidia) and Pestalotiopsis (4 isolates with concolorous conidia).
机译:普通的番石榴(Psidiwn guajava l.)是全球经济的果树。它在亚洲,南和中美洲种植,夏威夷精致的香气和味道,营养和医疗特性。然而,番石榴产量受番石榴痂的限制,由Pestalotiopsis属的真菌引起。番石榴痂的特征症状是果实表面上的皮层,卵形或圆形病变。这些病变可能变厚,影响以下肉体并降低果实质量和商业价值。我们以不同地区的番石榴叶和水果在番石榴叶片和水果上的蛋白皮叶和水果中分离出来的81个分离物,并将其作为Pestaloidsis和Neopestalotiopsis SPP鉴定。我们基于内部转录的间隔物,β-管蛋白和伸长因子基因的序列分析了分离物的形态学,致病性和遗传多样性。分离物在形态学上,病原体和遗传多样化,但多样性与地理来源没有相关,或者从中回收分离物的番石榴品种或组织。在多基因分析中包含的选定的单体分离物被鉴定为属于两个属:Neopestalotiopsis(65分离物,含有多元的分枝胞)和荚膜(4分离物与色谱分离蛋白)。

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