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首页> 外文期刊>Plant Physiology and Biochemistry >Anthocyanin accumulation and biosynthesis are modulated by regulated deficit irrigation in Cabernet Sauvignon (Vitis Vinifera L.) grapes and wines
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Anthocyanin accumulation and biosynthesis are modulated by regulated deficit irrigation in Cabernet Sauvignon (Vitis Vinifera L.) grapes and wines

机译:通过Cabernet Sauvignon(葡萄vinifera L.)葡萄和葡萄酒的调节缺陷灌溉调节花青素积累和生物合成

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Anthocyanins contents and compositions play an important role in grape berries and wines. Grapevines are widely cultivated in arid and semi-arid areas, and water shortage restricts the development of wine industry. The aim of this work was to gain insight on the effect of regulated deficit irrigation (RDI) on the accumulation and biosynthesis of anthocyanins in Cabernet Sauvignon (Vitis Vinifera L.) grapes and wines. High-performance liquid chromatography (HPLC) was used for anthocyanins profiles analyses and real-time quantitative PCR (qRT-PCR) was used for the genes expressions measurement. The grapevines were treated with 60% (RDI-1), 70% (RDI-2), 80% (RDI-3), 100% (CK, traditional drip irrigation) of their estimated evapotranspiration (ETc) respectively. RDI treatments significantly reduced titration acid and increased pH with higher total soluble solids. RDI-1 treatment increased total anthocyanins contents in berries and wines in both two vintages. RDI-1 and RDI-2 treatments significantly increased the contents of acylated anthocyanins in berries and wines, especially Malvidin-3-acetly-glucoside. RDI treatments significantly increased non-acylated anthocyanins contents in wines, such as Delphinidin-3-gliucoside and Malvidin-3-glucoside. RDI treatments upregulated the expressions of VvPAL, VvC4H, VvCHS, VvF3'H, VvF3'5'H, VvLDOX, and VvOMT in both two vintages. Correlation analysis showed the accumulation of anthocyanins was closely related to the key genes expressions, including VvPAL, VvF3'H, VvF3'5'H etc. The present results provided direct evidence and detailed data to explain that RDI treatments regulated the accumulation of anthocyanins by regulating genes expressions in the anthocyanin synthesis pathway.
机译:花青素含量和组合物在葡萄浆果和葡萄酒中起着重要作用。葡萄园在干旱和半干旱地区广泛培育,水资源短缺限制了葡萄酒行业的发展。这项工作的目的是深入了解受管制赤字灌溉(RDI)对赤葡萄酒(葡萄葡萄葡萄葡萄葡萄酒)葡萄和葡萄酒的分类和生物合成的影响。高效液相色谱(HPLC)用于花青素谱分析,使用实时定量PCR(QRT-PCR)用于基因表达测量。将葡萄葡萄葡萄化为60%(RDI-1),70%(RDI-2),80%(RDI-3),100%(CK,传统滴灌)分别的估计蒸发(ETC)。 RDI治疗显着降低滴定酸,并具有较高的总可溶性固体的pH值。 RDI-1治疗在两年葡萄酒中增加了浆果和葡萄酒中的总花青素含量。 RDI-1和RDI-2治疗显着增加了浆果和葡萄酒中酰基化花青素的含量,特别是麦类-3-乙糖苷。 RDI治疗显着增加了葡萄酒中的非酰基化的花青素含量,例如林蛋白-3-巯基和麦芽蛋白-3-葡糖苷。 RDI治疗上调了VVPAL,VVC4H,VVCHS,VVF3'H,VVF3'5'H,VVLDOX和VVOMT的表达式。相关性分析表明,花青素的积累与关键基因表达密切相关,包括VVPAL,VVF3'H,VVF3'5'H等。目前的结果提供了直接证据和详细数据,以解释RDI治疗中的分配受到花青素的积累调节花青素合成途径中的基因表达。

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