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首页> 外文期刊>Plant Physiology and Biochemistry >Suppression of the beta-carotene hydroxylase gene increases beta-carotene content and tolerance to abiotic stress in transgenic sweetpotato plants Le Kang
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Suppression of the beta-carotene hydroxylase gene increases beta-carotene content and tolerance to abiotic stress in transgenic sweetpotato plants Le Kang

机译：抑制β-胡萝卜素羟化酶基因的β-胡萝卜素含量增加和对转基因甘薯植物中非生物胁迫的耐受性

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摘要

beta-carotene, a carotenoid that plays a key photo-protective role in plants is converted into zeaxanthin by a beta-carotene hydroxylase (CHY-beta). Previous work showed that down-regulation of IbCHY-beta by RNA interference (RNAi) results in higher levels of beta-carotene and total carotenoids, as well as salt stress tolerance, in cultured transgenic sweetpotato cells. In this study, we introduced the RNAi-IbCHY-beta construct into a white-fleshed sweetpotato cultivar (cv. Yulmi) by Agrobacterium-mediated transformation. Among the 13 resultant transgenic sweetpotato plants (referred to as RC plants), three lines were selected for further characterization on the basis of IbCHY-beta transcript levels. The RC plants had orange flesh, total carotenoid and beta-carotene contents in storage roots were 2-fold and 16-fold higher, respectively, than those of non-transgenic (NT) plants. Unlike storage roots, total carotenoid and beta-carotene levels in the leaves of RC plants were slightly increased compared to NT plants. The leaves of RC plants also exhibited tolerance to methyl viologen (MV)-mediated oxidative stress, which was associated with higher 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical beta-scavenging activity. In addition, RC plants maintained higher levels of chlorophyll and higher photosystem II efficiency than NT plants after 250 mM NaCI stress. Yield of storage roots did not differ significantly between RC and NT plants. These observations suggest that RC plants might be useful as a nutritious and environmental stress -tolerant crop on marginal lands around the world. (C) 2017 Elsevier Masson SAS. All rights reserved.
机译：β-胡萝卜素，在植物中发挥关键照片保护作用的类胡萝卜素被β-胡萝卜素羟化酶（Chy-β）转化为Zeaxanthin。以前的作品表明，通过RNA干扰（RNAi）对IBCHY-β的下调导致培养的转基因甜菜藻细胞中的β-胡萝卜素和总类胡萝卜素和总类胡萝卜素以及盐胁迫耐受性。在这项研究中，我们通过农杆菌介导的转化将RNAi-Ibchy-β构建成白皮甘薯栽培品种（CV.yulmi）。在13种所得的转基因甘薯植物中（称为RC植物）中，选择三条线以基于IBCHY-Beta转录水平进行进一步表征。 RC植物具有橙色肉，储存根的总类胡萝卜素和β-胡萝卜素含量分别比非转基因（NT）植物的2倍和16倍。与NT植物相比，与储存根和RC植物叶片中的总类胡萝卜素和β-胡萝卜素水平略微增加。 RC植物的叶片还表现出对甲基Viologen（MV）介导的氧化应激的耐受性，其与更高的2,2-二苯基-1-富铬酰基（DPPH）基团β-清除活性相关。此外，RC植物在250mM NaCl应力后，RC植物保持较高水平的叶绿素和更高的照相效率，而不是NT植物。 RC和NT植物之间的储存根源的产量没有显着差异。这些观察结果表明，RC植物可能被视为世界各地边缘土地上的营养和环境压力 - 营养作物。 （c）2017年Elsevier Masson SAS。版权所有。

著录项

  • 来源
    《Plant Physiology and Biochemistry》 |2017年第2017期|共10页
  • 作者

    Kang Le; Ji Chang Yoon; Kim Sun Ha; Ke Qingbo; Park Sung-Chul; Kim Ho Soo; Lee Hyeong-Un; Lee Joon Seol; Park Woo Sung; Ahn Mi-Jeong; Lee Haeng-Soon; Deng Xiping; Kwak Sang-Soo;

  • 作者单位

    Korea Res Inst Biosci &

    Biotechnol Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    Korea Res Inst Biosci &

    Biotechnol Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    Korea Res Inst Biosci &

    Biotechnol Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    Korea Res Inst Biosci &

    Biotechnol Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    Korea Res Inst Biosci &

    Biotechnol Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    Korea Res Inst Biosci &

    Biotechnol Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    Rural Dev Adm Natl Inst Crop Sci Bioenergy Crop Res Inst 199 Muan Ro Muan Gun 58545 South Korea;

    Rural Dev Adm Natl Inst Crop Sci Bioenergy Crop Res Inst 199 Muan Ro Muan Gun 58545 South Korea;

    Gyeongsang Natl Univ Coll Pharm 501 Jinjudae Ro Jinju 52828 South Korea;

    Gyeongsang Natl Univ Coll Pharm 501 Jinjudae Ro Jinju 52828 South Korea;

    Korea Res Inst Biosci &

    Biotechnol Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

    Northwest A&

    F Univ Inst Soil &

    Water Conservat State Key Lab Soil Eros &

    Dryland Farming Loess P Xianyang Shaanxi Peoples R China;

    Korea Res Inst Biosci &

    Biotechnol Plant Syst Engn Res Ctr 125 Gwahak Ro Daejeon 34141 South Korea;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 植物生理学;植物生物化学;
  • 关键词

    beta-carotene hydroxylase; Carotenoid; Oxidative stress; RNA interference; Salt stress; Sweetpotato;

    机译：β-胡萝卜素羟化酶;类胡萝卜素;氧化胁迫;RNA干扰;盐胁迫;甘薯;

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