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首页> 外文期刊>Plant Protection Science >Virulence of new strain of Heterorhabditis bacteriophora from Croatia against Lasioptera rubi
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Virulence of new strain of Heterorhabditis bacteriophora from Croatia against Lasioptera rubi

机译：克罗地亚克罗地亚克罗地亚对克罗地亚鲁西的毒力毒力

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Soil samples from 100 cultivated and natural sites were assessed for the presence of entomopathogenic nematodes. Heterorhabditid nematodes were recovered from three soil samples during spring months, with the overall positive sample rate of 3%. The isolates of entomopathogenic nematodes were identified as three different strains conspecific with Heterorhabditis bacteriophora (I leterorhabditidae). They were found from natural sites and vineyard, while no recovery occurred from intensively cultivated agricultural fields. The morphometrical characteristics of infective juveniles and males showed differences between all Croatian strains and from the original description. Heterorhabditis bacteriophora ISO9 was bioassayed on Lasioptera rubi (Cecidomyiidae) (the raspberry gall midge) larvae at different nematode concentrations under laboratory conditions. The significantly highest mortality was observed in treatments with 50 and 200 infective juveniles per insect larvae within 8 days after inoculation. This is the first report of entomopathogenic nematodes of the family Heterorhabditidae from Croatia, and susceptibility of L. rubi larvae to entomopathogenic nematodes. The Croatian strain H. bacteriophora ISO9 was proved to possess strong insecticidal properties against L. rubi larvae.
机译：评估来自100种栽培和天然部位的土壤样品用于昆虫致血致原性线虫的存在。在春季期间从三种土壤样品中回收异激虫线虫，总体阳性样品率为3％。诱导昆虫致病线虫的分离物被鉴定为三种不同的菌株与杂菌炎细菌（I Leterorhabditidae）的特异性。它们是从天然遗址和葡萄园中发现的，同时没有培养农业领域的恢复。感染幼稚和雄性的形态学特性在所有克罗地亚菌株和原始描述之间表现出差异。在实验室条件下，杂种肝炎菌在不同线虫浓度下的Lasioptera鲁西（Cecidomyiidae）（覆盆子静脉）幼虫（覆盆子静脉）幼虫。在接种后8天内，在每虫幼虫50和200个感染幼年胞的治疗中观察到显着最高的死亡率。这是来自克罗地亚的家族杂种疗法线虫的第一报告，以及L.Rubi幼虫对受昆虫致病线虫的易感性。克罗地亚菌株H. Bacteriophora ISO9被证明对L. Rubi幼虫具有强烈的杀虫性能。

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