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首页> 外文期刊>Plant Science: An International Journal of Experimental Plant Biology >Suppression of a hexokinase gene, SlHXK1, leads to accelerated leaf senescence and stunted plant growth in tomato
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Suppression of a hexokinase gene, SlHXK1, leads to accelerated leaf senescence and stunted plant growth in tomato

机译：抑制六酮酶基因，SlHXK1，导致番茄中的加速叶片衰老和植物生长发育不良

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摘要

Sugars are the key regulatory molecules that impact diverse biological processes in plants. Hexokinase, the key rate-limiting enzyme in hexose metabolism, takes part in the first step of glycolytic pathway. Acting as a sensor that mediates sugar regulation, hexokinase has been proved to play significant roles in regulating plant growth and development. Here, we isolated a hexokinase gene SlHXKI from tomato. Its transcript levels were higher in flowers and leaves than in other organs and decreased during leaf and petiole development. SlHXKI-RNAi lines displayed advanced leaf senescence and stunted plant growth. Physiological features including plant height, leaf length, thickness and size, the contents of chlorophyll, starch and MDA, and hexokinase activity were dramatically altered in SlHXKI-RNAi plants. Dark-induced leaf senescence were advanced and the transcripts of senescence-related genes after darkness treatment were markedly increased in SlHXKI-RNAi plants. RNA-seq and ciRT-PCR. analyses showed that the transcripts of genes related to plant hormones, photosynthesis, chloroplast development, chlorophyll synthesis and metabolism, cellular process, starch and sucrose metabolism, and senescence were significantly altered in SlHXKI-RNAi plants. Taken together, our data demonstrate that SlHXKI is a significant gene involved in leaf senescence and plant growth and development in tomato through affecting starch turnover.
机译：糖是影响植物中不同生物过程的关键调节分子。 Hexokinase，Hexose代谢中的关键率限制酶，参与了糖酵解途径的第一步。作为调节糖调节的传感器，已被证明在调节植物生长和发育方面发挥显着作用。在这里，我们从番茄中孤立六酮酶基因Slhxki。它的转录水平在鲜花和叶子中比其他器官叶片更高，并且在叶子和叶柄发育过程中减少。 SLHXKI-RNAI线显示先进的叶片衰老和植物生长。在SLHXKI-RNAI植物中，包括植物高度，叶子长度，厚度和尺寸，叶绿素，淀粉和MDA的含量和六酮酶活性的含量。在SLHXKI-RNAI植物中，深入治疗后的衰老相关基因的成绩和衰老相关基因的成绩单明显增加。 RNA-SEQ和CIRT-PCR。分析表明，在SLHXKI-RNAI植物中，与植物激素，光合作用，叶绿体发育，叶绿素合成和代谢，细胞过程，淀粉和蔗糖代谢有关的基因的转录物显着改变。我们的数据携带，证明Slhxki是番茄叶衰老和植物生长发育的重要基因，通过影响淀粉营业额。

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