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首页> 外文期刊>Plant Species Biology >Development and characterization of EST-SSR markers in an East Asian temperate plant genus Diabelia (Caprifoliaceae)
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Development and characterization of EST-SSR markers in an East Asian temperate plant genus Diabelia (Caprifoliaceae)

机译：东亚温带植物属苜蓿（Caprifoliaceae）中EST-SSR标记的开发与鉴定

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摘要

A set of expressed sequence tag (EST) simple sequence repeat (SSR) markers were developed and characterized using next-generation sequencing technology for the genus Diabelia (Caprifoliaceae). De novo assembly of RNA-seq reads resulted in 58669 contigs with the N50 length of 1211bp. A total of 2746 contigs were identified to harbor SSR motifs, of which 48 primer pairs were designed and 11 were shown to be polymorphic across three morphospecies of Diabelia. When evaluated with 30 individuals, the number of alleles per locus ranged from 2 to 11 and the expected heterozygosity varied from 0.399 to 0.873, respectively. Distance-based clustering indicated that the EST-SSR markers can provide sufficient power to distinguish the three species (or populations). These markers will be useful for evaluating the range-wide genetic diversity of each species and examining genetic divergence and gene flow between the three species.
机译：开发了一组表达的序列标签（EST）简单序列重复（SSR）标记，并使用下一代测序技术进行糖尿病（Caprifoliaceae）的下一代测序技术。 RNA-SEQ读取的DE Novo组装导致58669个Contig，N50长度为1211bp。 鉴定了总共2746个ContIG，以涉及SSR基序，其中设计了48个引物对，并且在糖尿病的三种形态学中显示出11种多态性。 当用30个个体进行评估时，每个基因座的等位基因数量为2至11，预期的杂合子分别从0.399变化到0.873。 基于距离的聚类表明EST-SSR标记可以提供足够的力量来区分三种物种（或群体）。 这些标记对于评估每种物种的范围范围的遗传多样性并检查三种物种之间的遗传分歧和基因流动。

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