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首页> 外文期刊>Planta: An International Journal of Plant Biology >Auxin acts as a downstream signaling molecule involved in hydrogen sulfide-induced chilling tolerance in cucumber
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Auxin acts as a downstream signaling molecule involved in hydrogen sulfide-induced chilling tolerance in cucumber

机译：植物蛋白作为下游信号分子，参与黄瓜中的硫化氢诱导的冷却耐受性

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Main conclusion This report proves a cross talk between H2S and IAA in cold stress response, which has presented strong evidence that IAA acts as a downstream signal mediating the H2S-induced stress tolerance in cucumber seedlings. We evaluated changes in endogenous hydrogen sulfide (H2S) and indole-3-acetic acid (IAA) emission systems, and the interactive effect of exogenous H2S and IAA on chilling tolerance in cucumber seedlings. The results showed that chilling stress increased the activity and relative mRNA expression of l-/d-cysteine desulfhydrase (l-/d-CD), which in turn induced the accumulation of endogenous H2S. Similarly, the endogenous IAA system was triggered by chilling stress. We found that 1.0 mM sodium hydrosulfide (NaHS, an H2S donor) significantly enhanced the activity of flavin monooxygenase (FMO) and relative expression of FMO-like proteins (YUCCA2), which in turn elevated endogenous IAA levels in cucumber seedlings. However, IAA had little effects on activities of l-/d-CD and endogenous H2S levels. H2S-induced IAA production accompanied by increase in chilling tolerance, as shown by the decrease in stress-induced electrolyte leakage (EL) and reactive oxygen species (ROS) accumulation, and increase in gene expressions and enzyme activities of photosynthesis. 1-naphthylphthalamic acid (NPA, an IAA polar transport inhibitor) declined H2S-induced chilling tolerance and defense genes' expression. However, scavenging of H2S had a little effect on IAA-induced chilling tolerance. These results suggest that IAA acting as a downstream signaling molecule is involved in the H2S-induced chilling tolerance in cucumber seedlings.
机译：主要结论本报告证明了H2S和IAA之间的串联谈论冷应激反应，这提出了强大的证据，即IAA作为下游信号调解黄瓜幼苗中H2S诱导的应激耐受性。我们评估了内源性硫化氢（H2S）和吲哚-3-乙酸（IAA）排放系统的变化，以及外源H2S和IAA对黄瓜幼苗冷冻耐受的交互作用。结果表明，寒冷应力增加了L- / D-半胱氨酸脱硫酶（L- / D-CD）的活性和相对mRNA表达，其又诱导了内源H2S的积累。同样，通过冷却应力引发内源性IAA系统。我们发现1.0mM氢硫化钠（NaHS，H 2 S供体）显着提高了黄素单氧化酶（FMO）的活性和FMO样蛋白（YUCCA2）的相对表达，其在黄瓜幼苗中呈升高的内源性IAA水平。然而，IAA对L-/ D-CD和内源H2S水平的活动几乎没有影响。 H2S诱导的IAA生产伴随着冷却耐受性的增加，如胁迫诱导的电解质泄漏（EL）和反应性氧物质（ROS）积累的降低所示，以及基因表达的增加和光合作用的酶活性。 1-萘氨基苯甲酸（NPA，IAA极性传输抑制剂）下降了H2S诱导的冷却耐受性和防御基因的表达。然而，H2S的清除对IAA引起的冷却耐受性有点影响。这些结果表明IAA作为下游信号分子的作用涉及黄瓜幼苗的H2S诱导的冷却耐受性。

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