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首页> 外文期刊>Plant Pathology >Ralstonia solanacearum preferential colonization in the shoot apical meristem explains its pathogenicity pattern in tomato seedlings
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Ralstonia solanacearum preferential colonization in the shoot apical meristem explains its pathogenicity pattern in tomato seedlings

机译：Ralstonia solanacearum优先殖民化在拍摄顶端公司中解释了番茄幼苗的致病性模式

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摘要

Ralstonia solanacearum causes a lethal bacterial wilt disease in many plants by colonizing the vascular tissues of the hosts. Upon inoculation of tomato seedlings through either leaf or root, the wilting symptoms occur first at the apical region and then proceed downward along the shoot. The systemic order of the disease initiation and progression in the host, independent of the site of pathogen inoculation, is yet to be investigated. To understand the disease progression more clearly, we have carried out a systematic study of the pathogen localization by GUS staining of inoculated tomato seedlings, at 24-hour intervals from 0 days post-inoculation (dpi) to 5 dpi. In both inoculation methods, pathogen colonization was observed at 1 dpi at the apical meristem as well as the cotyledon leaves, where the disease initiates. As the disease progressed, colonization by the pathogen towards the lower region of the shoot was observed. Disease consistency and pathogenicity magnitude were observed to be higher using the leaf inoculation method than the root inoculation method. Several R. solanacearum transposon-induced mutants that were reduced in virulence by root inoculation but virulent by leaf inoculation were obtained. Using GUS staining, it was observed that these mutants were unable to localize in the shoot region when inoculated in the root. Our study indicates that the apical meristem and the cotyledon leaves are the first regions to be colonized in inoculated tomato seedlings, which might explain the disease initiation from this region.
机译：Ralstonia Solanacearum通过定植宿主的血管组织来引起许多植物中的致命细菌枯萎病。通过叶片或根部接种番茄幼苗后，首先在顶端区域发生枯萎的症状，然后沿着拍摄向下进行。尚未研究宿主的疾病引发和进展的全身秩序，尚未研究病症。为了更清楚地了解疾病的进展，我们对从接种后0天（DPI）至5 dpi的24小时间隔以24小时间隔进行了对植物植物的病原体定位的系统研究。在接种方法中，在Apick Meristem的1 dPI下观察到病原体结肠，以及疾病引发的子叶叶。随着疾病的进展，观察到病原体朝向芽的下部区域的定植。观察疾病一致性和致病性幅度使用叶片接种方法比根部接种方法更高。几种R.Solanacearum转座子诱导的突变体，通过根部接种而减少毒力，但是通过叶片接种进行毒力。使用GUS染色，观察到当接种在根部时，这些突变体不能在枝条区域中定位。我们的研究表明，在接种番茄幼苗中，顶端分泌物和子叶叶是第一个地区，其可能解释该区域的疾病启动。

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