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首页> 外文期刊>Plant Pathology >Functional analysis of walnut polyphenol oxidase gene (JrPPO1) in transgenic tobacco plants and PPO induction in response to walnut bacterial blight
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Functional analysis of walnut polyphenol oxidase gene (JrPPO1) in transgenic tobacco plants and PPO induction in response to walnut bacterial blight

机译：转基因烟草植物中核桃多酚氧化酶基因（JRPPO1）的功能分析及PPO诱导核桃细菌枯萎病

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Walnut (Juglans regia) is economically important for both its wood and nut nutritional value, but it is susceptible to diseases such as walnut bacterial blight, caused by Xanthomonas arboricola pv. juglandis (Xaj). Walnuts contain many phenolic compounds, providing a good model on which to study polyphenol oxidase (PPO). We inoculated the detached walnut fruits of cultivars Ford, Chandler, Franquette, Robert Livermore, and Payne with Xaj and measured the induction of PPO activity in infected sites and adjacent to infected sites. Compared to infected and uninfected sites, PPO activity was induced significantly in areas adjacent to infected sites in all cultivars except Ford. Ford and Franquette, presenting the lowest and highest PPO activity, showed the largest and smallest mean diameter spots in response to Xaj, respectively. Polyacrylamide gel electrophoresis confirmed monophenol oxidase activity of walnut PPO in the assessed tissues. Then, we revealed the antipathogenic potential of walnut PPO through Agrobacterium tumefaciens-mediated walnut JrPPO1 gene transfer into tobacco (Nicotiana tabacum). Two transformed tobacco lines overexpressing the JrPPO1 gene were regenerated successfully and challenged with Pseudomonas syringae pv. tabaci. Transgenic lines showed significantly higher PPO activity and lower disease severity to the pathogen compared to the control. However, a significant difference in disease severity and PPO activity level was observed between the two transgenic lines. Our results demonstrate a potential defence-related role of PPO in transgenic tobacco and its induction in areas adjacent to infection sites in walnut cultivars treated with Xaj.
机译：核桃（Juglans Regia）对其木材和螺母营养价值是经济的重要性，但它易受核桃细菌枯萎病等疾病的影响，由Xanthomonas植物植物植物PV引起。 juglandis（xaj）。核桃含有许多酚类化合物，提供了研究多酚氧化酶（PPO）的良好模型。我们用XAJ接种了品种福特，钱德勒，Franquette，Robert Livermore和Payne的独立核桃果实，并测量了感染遗址的PPO活性，邻近受感染的地方。与感染和未感染的位点相比，除了福特以外的所有品种的受感染位点相邻的地区，PPO活性会显着诱导。福特和Franquette分别显示最低和最高的PPO活动，显示出响应XAJ的最大和最小的平均直径点。聚丙烯酰胺凝胶电泳证实了评估组织中核桃PPO的单选酚氧化酶活性。然后，我们揭示了通过Tumefaciens介导的核桃JRPPO1基因转移到烟草（Nicotiana Tabacum）中的核桃PPO的抗疟潜力。过表达JRPPO1基因的两个转化的烟草线被成功再生，并用假单胞菌Syringae PV攻击。 Tabaci。与对照相比，转基因系显示出明显较高的PPO活性和降低疾病严重程度。然而，在两种转基因系之间观察到疾病严重程度和PPO活性水平的显着差异。我们的结果表明，PPO在转基因烟草中的潜在防御相关的作用及其在用XAJ处理的核桃品种的感染部位附近的区域诱导。

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