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首页> 外文期刊>BJU international >Muscle precursor cell autografting in a murine model of urethral sphincter injury.
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Muscle precursor cell autografting in a murine model of urethral sphincter injury.

机译：在尿道括约肌损伤的小鼠模型中自体肌肉前体细胞移植。

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OBJECTIVE: To determine whether muscle precursor cells (MPCs) harvested from limb skeletal muscle can enhance the regeneration process of the striated urethral sphincter after injury. MATERIAL AND METHODS: Striated urethral sphincters of male mice were injured by an injection of a myotoxic substance (notexin). In the experimental group, 2 days after injury, MPCs were enzymatically harvested from striated muscles of the lower limbs and labelled with PKH 26, then immediately re-injected into the injured urethral sphincter of the same animal. In the control group, saline buffer was injected instead of MPCs. Animals were killed 7 days or 1 month after injury and the sphincters removed for histological study (the presence of PKH 26-labelled myofibres, measurement of myofibre diameter and total number of myofibres). RESULTS: MPC autografting accelerated sphincter muscle repair, as shown by a higher myofibre diameter (P = 0.03) and number (P = 0.01) in the experimental group than in the controls at 7 days. One month after their injection MPCs were still detectable in the regenerating sphincters and participated in the formation of new myofibres. CONCLUSION: This study provides the experimental basis for a new therapeutic approach to urethral sphincter insufficiency after surgical or obstetrical injury, based on MPC autografting.
机译：目的：确定从肢体骨骼肌中收获的肌肉前体细胞（MPC）是否可以增强损伤后横纹尿道括约肌的再生过程。材料和方法：雄性小鼠的横纹尿道括约肌通过注射肌毒性物质（notexin）而受伤。在实验组中，在受伤后2天，从下肢的横纹肌中酶促收集MPC并用PKH 26标记，然后立即再次注射到同一动物的受伤的尿道括约肌中。对照组注射生理盐水代替MPCs。损伤后7天或1个月处死动物，取出括约肌进行组织学研究（PKH 26标记的肌纤维的存在，肌纤维直径的测量和肌纤维总数）。结果：MPC自体移植加速了括约肌修复，如第7天，实验组的肌纤维直径（P = 0.03）和数量（P = 0.01）比对照组更高。注射一个月后，仍在再生括约肌中检测到MPC，并参与了新的肌纤维的形成。结论：本研究为基于MPC自体移植的外科或产科损伤后尿道括约肌功能不全的新治疗方法提供了实验依据。

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