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首页> 外文期刊>Physiology and Molecular Biology of Plants >Identification of candidate resistance genes of cotton against Aspergillus flavus infection using a comparative transcriptomics approach
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Identification of candidate resistance genes of cotton against Aspergillus flavus infection using a comparative transcriptomics approach

机译：使用比较转录组学方法鉴定棉花患者对曲霉感染的抗性基因

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摘要

A comparative transcriptome analysis was performed using the genes significantly differentially expressed in cotton, corn and peanut in response to aflatoxin producing fungus Aspergillus flavus with an objective of identifying candidate resistance genes in cotton. Two-way analyses identified 732 unique genes to be differentially regulated by the fungus with only 26 genes common across all three crops that were considered candidate A. flavus resistance genes with an assumption that these genes have specific roles in conferring the resistance trait. Genes of membrane cellular component involved in DNA binding with involvement in defense responses were highly represented among the differentially expressed unique genes. Most (six) of these genes coded for 2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily proteins. Genes encoding helix loop helix protein, alcohol dehydrogenase and UDP glycosylation transferase which were upregulated in response to both atoxigenic and toxigenic strains of A. flavus, could be potential resistance candidate genes for downstream functional manipulation to confer resistance.
机译：使用棉花，玉米和花生显着表达的基因进行比较转录组分析，响应于黄曲霉毒素产生真菌曲霉属植物，其目的是鉴定棉花中的候选抗性基因。双向分析确定了732个独特的基因，该基因由所有三种作物中常见的真菌进行差异调节，这些作物被认为是候选A.患者抗性基因的假设，即这些基因在赋予抗性特征方面具有特异性作用。在差异表达的独特基因中，高度表示与差异反应的DNA结合中涉及的膜细胞组分的基因。大多数（六个）这些基因编码为2-氧氧化术（2g）和Fe（II） - 依赖性氧合酶超家族蛋白。编码螺旋环螺旋蛋白，醇脱氢酶和UDP糖基化转移酶的基因，其响应于A.FlaVus的毒性和毒性毒株而上调，可以是用于下游功能操纵以赋予抵抗的潜在抗性候选基因。

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