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首页> 外文期刊>Phytochemical Analysis >A feasible UHPLC-MS/MS method for concurrent quantification of 10 bioactive principles in Aquilaria leaf tea by the multiple reaction monitoring analytical mode
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A feasible UHPLC-MS/MS method for concurrent quantification of 10 bioactive principles in Aquilaria leaf tea by the multiple reaction monitoring analytical mode

机译：通过多重反应监测分析模式对Aquilaria叶片茶中10种生物活性原理的同时量化的可行性UHPLC-MS / MS方法

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Introduction Recently, the fresh leaves of Aquilaria trees have been processed as food products such as agarwood tea due to its beneficial medicinal properties. However, there have not been any reported analytical methods to quantify the bioactive principles in the processed products. Objective A rapid and sensitive ultrahigh-performance liquid chromatography (UHPLC) coupled with electrospray ionisation (ESI) tandem mass spectrometry (MS/MS) method was developed and validated for the simultaneous determination of 10 bioactive components in Aquilaria leaf tea. Methods The UHPLC-MS/MS was used for quantification operated in multiple reaction monitoring (MRM) mode. The optimised chromatographic parameters were conducted on a Shim-pack XR-ODS II column and mobile phases consisted of acetonitrile and 0.1% formic acid in water. Results All the samples were analysed within 20 min. The established method showed excellent linearity (R-2 > 0.9988), good repeatability with all the relative standard deviation values lower than 3.27%, and satisfactory recovery (79.72-119.22%). The matrix effect factors ranged from 87.65 to 97.27% in the examination. The developed method was applied to the determination of 10 bioactive principles (1-10) in six different Aquilaria leaf tea samples. Among the analytes, mangiferin (1) and iriflophenone 2-O-alpha-l-rhamnopyranoside (2) were the most abundant compounds in the extracts of Aquilaria leaf tea, and it indicated that these biotech products may possess laxative effects. Conclusion This proposed method appeared to be a useful tool for the quality control of commercial products of Aquilaria leaf tea and thus provided an analytical reference for herbal drinks.
机译：介绍最近，由于其有益的药物性质，Aquilaria树的新鲜叶子已被加工为伞状茶等食品。然而，没有任何报告的分析方法可以量化加工产品中的生物活性原则。目的是一种快速敏感的超高性能液相色谱（UHPLC）与电喷雾电离（ESI）串联质谱（MS / MS）方法进行开发并验证，同时测定Aquilaria叶茶中的10种生物活性成分。方法采用UHPLC-MS / MS用于多重反应监测（MRM）模式的定量。优化的色谱参数在垫片XR-ODS II柱和移动相中进行，由乙腈和0.1％甲酸组成。结果在20分钟内分析所有样品。所建立的方法显示出优异的线性度（R-2> 0.9988），可重复性良好，所有相对标准偏差值低于3.27％，令人满意的恢复（79.72-119.22％）。矩阵效应因子在考试中的87.65％至97.27％。将开发方法应用于六种不同Aquilaria叶茶样品中10种生物活性原理（1-10）的测定。在分析物中，Mangiferin（1）和Iriflophyone 2-O-α-L- rhamyranide（2）是Aquilaria叶茶提取物中最丰富的化合物，表明这些生物技术产品可能具有泻药。结论这一提出的方法似乎是Aquilaria叶茶商业产品质量控制的有用工具，从而为草药饮料提供了分析参考。

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