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首页> 外文期刊>Polymers for advanced technologies >Preparation and antimicrobial properties of LL-37 peptide immobilized lignin/caprolactone polymer film
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Preparation and antimicrobial properties of LL-37 peptide immobilized lignin/caprolactone polymer film

机译：L1-37肽固定的木质素/己内酯聚合物膜的制备和抗微生物性能

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摘要

The use of biopolymers has gained priority in tissue engineering and biotechnology, both as dressing material and for enhancing treatment efficiency. There is a demand for new biopolymers designed with protease inhibitors and antimicrobials. LL-37 is an important antimicrobial peptide in human skin and exhibits a broad spectrum of antimicrobial activity against bacteria, fungi, and viral pathogens. Using lignin which is an abundant carbohydrate polymer in nature and a polyacrylic acid, we prepared a lignin/caprolactone biodegradable film by plastifying caprolactone and polyacyrlic acid. Lignin/caprolactone biodegradable film was activated with CDI and then immobilized LL-37 peptide. The structure was elucidated in terms of its functional groups by attenuated total reflectance-fourier transform infrared spectroscopy (ATR-FTIR), and the morphology of the lignin/caprolactone biodegradable film was characterized by scanning electron microscopy (SEM) before and after the immobilization process. The amount of LL-37 immobilized was determined by ELISA method. It was found that 97% of LL-37 peptide was successfully immobilized onto the lignin/caprolactone biodegradable film. Antimicrobial activity was determined in the lignin/caprolactone biodegradable film samples by quantitative antimicrobial activity method. According to the results, LL-37 immobilized lignin/caprolactone biodegradable film samples were effective on test organisms; Gram-positive Staphylococcus aureus and Gram-negative Escherichia coli. In bio-compatibility assays, the ability to support tissue cell integration was detected by using 3 T3 mouse fibroblasts. Samples were examined under transverse microscope, non-immobilized sample showed a huge cellular death, whereas LL-37 immobilized lignin/caprolactone biodegradable film had identical cellular growth with the control group. This dual functional lignin/caprolactone biodegradable film with enhanced antibacterial properties and increased tissue cell compatibility may be used to design new materials for various types of biological applications.
机译：生物聚合物的使用在组织工程和生物技术中获得了优先级，无论是敷料材料，都为加剧材料和加强治疗效率。需要采用蛋白酶抑制剂和抗微生物设计的新型生物聚合物。 LL-37是人体皮肤中重要的抗微生物肽，并具有针对细菌，真菌和病毒病原体的广谱抗菌活性。使用本质上是一种丰富的碳水化合物聚合物和聚丙烯酸，通过使己内酯和聚酰丙酸制备木质素/己内酯可生物降解薄膜。用CDI活化木质素/己内酯可生物降解薄膜，然后固定化LL-37肽。通过减毒的总反射率 - 傅里叶变换红外光谱（ATR-FTIR）而阐明该结构，并且通过在固定过程之前和之后扫描电子显微镜（SEM），表征木质素/己内酯可生物降解膜的形态。 。通过ELISA方法测定固定的LL-37的量。发现97％的LL-37肽被成功地固定在木质素/己内酯可生物降解膜上。通过定量抗微生物活性法测定木质素/己内酯可生物降解薄膜样品中的抗菌活性。根据结果​​，LL-37固定化木质素/己内酯可生物降解薄膜样品对测试生物有效;革兰氏阳性金黄色葡萄球菌和革兰氏阴性大肠杆菌。在生物相容性测定中，通过使用3 T3小鼠成纤维细胞检测支持组织细胞积分的能力。在横向显微镜下检查样品，非固定化样品显示出巨大的细胞死亡，而LL-37固定化的木质素/己内酯可生物降解薄膜与对照组具有相同的细胞生长。这种双功能性木质素/己内酯可生物降解膜具有增强的抗菌性能和增加的组织细胞相容性可用于为各种类型的生物应用设计新材料。

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