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PANi/PAN copolymer as scaffolds for the muscle cell-like differentiation of mesenchymal stem cells

机译：PANI / PAN共聚物作为间充质干细胞的肌肉细胞样分化的支架

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Nowadays, regeneration medicine or skeletal muscle tissue engineering can be a useful solution for the replacement of damaged or lost tissue. This study was carried out to evaluate the human bone marrow-derived mesenchymal stem cells differentiated into muscle-like cells on polyaniline (PANi)/polyacrylonitrile (PAN) copolymer conductive. After electrospinning of biocompatible PANi/PAN copolymers, they were modified by non-thermal oxygen plasma. The scaffold was characterized by scanning electron microscope (SEM), contact angle, and attenuated total reflectance-Fourier transform infrared spectroscopy. Cytocompatibility and viability of mouse fibroblast cells and mesenchymal stem cells were conducted by 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide assay. 4,6-Diamidino 2-phenylindole and SEM tests were performed to determine the cell survival on scaffolds. Reverse transcription polymerase chain reaction and immunocytochemistry assay performed to ensure that mesenchymal stem cells differentiated into muscle-like cells. SEM images of PANi/PAN nanofibers, contact angle, and attenuated total reflectance-Fourier transform infrared spectroscopy showed that oxygen-treated plasma scaffolds have more favorable hydrophilic structure. The test results of cell culture, 3-[4,5-dimethylthiazol-2yl]-2,5-diphenyl tetrazolium bromide assay, SEM, and 4,6-diamidino 2-phenylindole showed that the cells have proliferated well on the scaffolds and have good adhesion, so nanofiber could support the cellular growth. After 21 days of induction of muscle differentiation, expressions of alpha-ACTININ, MYOSIN, and MYOGENIN genes were confirmed by reverse transcription polymerase chain reaction and expressions of M-cadherin and troponin were approved by immunocytochemistry test. According to our data, PANi/PAN scaffolds promoted and supported mesenchymal cells to muscle-like cells. Therefore, the scaffolds were able to perform as substrates for soft tissue engineering. Copyright (C) 2017 John Wiley & Sons, Ltd.
机译：如今，再生药物或骨骼肌组织工程可以是用于更换受损或丢失的组织的有用解决方案。进行该研究以评估分化成肌肉状细胞的人骨髓衍生的间充质干细胞（PANI）/聚丙烯腈（PAN）共聚物导电。在生物相容性PANI /锅共聚物的静电纺丝之后，通过非热氧等离子体改性它们。通过扫描电子显微镜（SEM），接触角和衰减的总反射率 - 傅里叶变换红外光谱，表征支架。小鼠成纤维细胞和间充质干细胞的细胞锁定和活力由3- [4,5-二甲基噻唑-200L] -2,5-二苯基四唑溴化物测定进行。进行4,6-二脒基2-苯基吲哚和SEM试验以确定支架上的细胞存活。逆转录聚合酶链反应和免疫细胞化学测定进行，以确保间充质干细胞分化成肌肉状细胞。 PANI / PAN纳米纤维的SEM图像，接触角和衰减总反射率 - 傅里叶变换红外光谱显示，氧处理的等离子体支架具有更有利的亲水结构。细胞培养的测试结果，3- [4,5-二甲基噻唑-200l] -2,5-二苯基四唑溴水解测定，SEM和4,6-二氨基2-苯基吲哚表示，细胞在支架上均匀良好具有良好的粘附性，因此纳米纤维可以支持细胞生长。在诱导肌肉分化诱导21天后，通过逆转录聚合酶链式反应确认α-肌醇蛋白，肌球蛋白，肌球蛋白和肌原素基因的表达，并通过免疫细胞化学测试批准M-Cadherin和肌钙蛋白的表达。根据我们的数据，PANI / PAN支架促进并支持间充质细胞以肌肉状细胞。因此，支架能够作为软组织工程作为基板。版权所有（c）2017 John Wiley＆Sons，Ltd。

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