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首页> 外文期刊>Poultry Science >Differential expression of water channel- and noncoding RNA biogenesis-related genes in three lines of chickens under a short-term water restriction
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Differential expression of water channel- and noncoding RNA biogenesis-related genes in three lines of chickens under a short-term water restriction

机译：短期水限制下三行鸡水通道和非分量RNA生物发生相关基因的差异表达

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Genetic selection for high growth rate has resulted in tremendous changes not only in feed efficiency, but also in water consumption between modern broilers and their ancestor jungle fowl (JF). However molecular mechanisms involved in water homeostasis are still not well defined. This study aimed, therefore, to determine the effect of short-term water restriction on the expression of water channel- and noncoding RNA biogenesis-related genes in the kidney and whole blood of JF, broiler population from the 1990s (RB1995), and modern broiler population developed in 2015 (ARB2015). Body weight-matched birds from each population were subjected to water restriction (WR) for 3 h or had ad libitum access to water in a 3 x 2 factorial design. The expression of target genes was determined by real-time quantitative PCR. WR significantly reduced body weight in RB1995, but not in JF or ARB2015. In the kidney, WR up-regulated the expression of AQP2 in all chicken populations, AQP3 in the RB1995, and ATP1B1 in JF and ARB2015. However, it down-regulated the expression of AQP4 in ARB2015 but had no effect on AVP expression. The expression of RNase III family enzymes also was altered by WR in a population-dependent manner, with DICER1 being down-regulated in JF and RB1995, Drosha was decreased in RB1995, and ARG2 was up-regulated in ARB2015. The expression of DGCR8 and TRBP1 was not affected by WR in any population; however, DGCR8 mRNA levels were significantly lower in RB1995 and ARB2015 compared to JF under both conditions. TRBP1 gene expression was significantly lower in RB1995 and ARB2015 compared to JF under WR conditions. In the blood, the expression of these genes also was altered by WR, but with different patterns than the kidney. The mRNA abundances of AQP, AVP, DICER1, DGCR8, AGO2, and TRBP1 were significantly decreased by WR in RB1995. However, the expression of AQP2, AVP, DGCR8, and TRBP1 was increased in WR-ARB2015 compared to the control. In the JF, there was no difference in the expression of these genes except for a significant up-regulation of TRBP1 in WR compared to the control group. To the best of our knowledge, this is the first report showing that water channels and the RNase III enzymes are differentially regulated by WR in a population-dependent manner, which may be due to differential postnatal growth and maturation. Their expression in the circulation could open new vistas for identification of new molecular signatures involved in adaptation to water-deprivation stress.
机译：高增长率的遗传选择导致巨大的变化不仅在饲料效率方面，也导致现代肉鸡和祖先丛林禽（JF）之间的耗水量。然而，涉及水稳态的分子机制仍然没有明确定义。因此，本研究旨在确定短期用水限制对肾脏和全血肾脏和全血的水通道和非划分的RNA生物发生相关基因的影响，从20世纪90年代（RB1995）和现代的肉鸡人口2015年开发的肉鸡人口（ARB2015）。每个人群的身体重量匹配的鸟类受到水限制（WR）3小时，或者在3 x 2因子设计中获得水的可利用。通过实时定量PCR测定靶基因的表达。 WR在RB1995中显着减少体重，但不在JF或ARB2015中。在肾脏中，WR上调所有鸡群中AQP2的表达，AQP3在RB1995中的AQP3和JF和ARB2015中的ATP1B1。然而，它下调AQP4在ARB2015中的表达，但对AVP表达没有影响。 RNase III家族酶的表达也以人群依赖性方式改变，DICER1在JF和RB1995中被下调，RB1995中的DOSHA降低，ARC2在ARB2015上调。 DGCR8和TRBP1的表达不受任何人口WR的影响;然而，与两个条件下的JF相比，RB1995和ARB2015中的DGCR8 mRNA水平显着降低。在WR条件下，RBP1基因表达在RB1995和ARB2015中显着降低。在血液中，这些基因的表达也被WR改变，但具有不同的模式而不是肾脏。 RB1995中WR，WR的AQP，AVP，DICER1，DGCR8，AGP1和TRBP1的mRNA丰富显着降低。然而，与控制相比，WR-ARB2015中AQP2，AVP，DGCR8和TRBP1的表达增加。在JF中，除了与对照组相比，这些基因表达除了对照组的TRBP1的显着上调外，没有差异。据我们所知，这是第一个报告，显示水通道和RNase III酶以人口依赖性方式差异地调节，这可能是由于差异的产前生长和成熟。它们在循环中的表达可以打开新的Vistas，用于鉴定适应水剥夺压力的新分子签名。

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