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首页> 外文期刊>Poultry Science >Bacterial communication between Lactobacillus spp. isolated from poultry in the inhibition of Salmonella Heidelberg-proof of concept
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Bacterial communication between Lactobacillus spp. isolated from poultry in the inhibition of Salmonella Heidelberg-proof of concept

机译：乳杆菌水合物之间的细菌通信。 从家禽被隔绝在抑制沙门氏菌海德堡证明的概念证明

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Bacterial communication has become an increasingly studied topic aiming at the discovery of new products to aid the treatment of diseases for which conventional options do not work. The production of safe foods, free of pathogens, has been receiving increasing attention due to market demands for food products of high quality and free of residues. This study assessed the communication between Lactobacillus spp. during the in vitro inhibition of Salmonella Heidelberg (SH) and the impact an autoinducer produced by a strain of Lactobacillus plantarum has on communication in the normal microbiota and inhibiting SH in newborn chicks. For this purpose, the isolates of Lactobacillus spp. were isolated cloacal swabs of broilers and identified through biochemical and molecular assays and were obtained from broiler farms. They later had their inhibitory potential against SH stimulated after contact with the autoinducer. For assessing bacterial communication (quorum sensing) during the inhibition of SH by Lactobacillus spp., a spot on the lawn assay was conducted. For the in vivo, 75 one-day-old chicks were divided in 5 experimental groups: control with no treatment; treatment with a Lactobacillus spp. pool on the first day; treatment with autoinducer on the second day; treatment with Lactobacillus spp. on the first day; and autoinducer on the second day and treatment without autoinducer. The autoinducer was assessed through an SH count in the ceca of the birds. The autoinducer produced by the strain of L. plantarum proved to be efficient for communicating with the other Lactobacillus spp. isolates as previous contact with SH induced the production of an autoinducer capable of increasing inhibition of SH both in vitro (in average 132.73%) and in vivo, acting similarly to the Lactobacillus spp. pool (probiotic) by decreasing the SH count in the ceca (64%-24 h, 42%-96 h, and 46%-168 h).
机译：细菌通信已成为一个越来越多的研究主题，旨在发现新产品，以帮助治疗常规选项不起作用的疾病。由于市场对高质量和不含残留物的食品需求，生产安全食品的生产，不断受到越来越关注。该研究评估了乳酸杆菌之间的沟通。在体外抑制沙门氏菌（Sh）和冲击乳杆菌菌株产生的胰腺产生的造影，在正常微生物群中的通信和抑制新生儿雏鸡。为此目的，乳杆菌SPP的分离物。被隔离的肉织物的肉织物拭子，并通过生化和分子测定鉴定，并从肉鸡农场获得。他们后来在与自动挤出机接触后对SH刺激的抑制潜力。用于评估乳酸杆菌抑制抑制过程中的细菌通信（法定感测）。，进行了草坪测定的斑点。对于体内，75人一日雏鸡分为5种实验组：对照无治疗;用乳酸杆菌治疗。第一天游泳池;第二天用自动化器治疗;用乳杆菌治疗SPP。在第一天;和自动化器在第二天和无自动化器的治疗。通过鸟类CECA的SH计数评估Auto uchiCer。由L.Portararum的菌株产生的自动挤出机被证明是与其他乳酸杆菌SPP汇流的有效。与先前接触的分离物与SH引起的胰岛素的产生能够增加体外（平均为132.73％）和体内抑制SH的抑制作用，与​​乳酸杆菌SPP类似地作用。通过减少CECA中的SH计数（64％-24小时，42％-96小时和46％-168小时）来池（益生菌）。

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