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首页> 外文期刊>Poultry Science >Protective effect of Salvia miltiorrhiza polysaccharides on liver injury in chickens
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Protective effect of Salvia miltiorrhiza polysaccharides on liver injury in chickens

机译：丹参对鸡肝损伤丹参的保护作用

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This study investigated the effects of Salvia miltiorrhiza polysaccharides (SMPs) on the injury of chicken hepatocytes in vitro and in vivo. In in vitro studies primary cultured hepatocytes were isolated by 2-step collagenase perfusion. Carbon tetrachloride (CCL4) was added to the hepatocytes to establish a hepatocyte injury model. Hepatocytes were treated with different concentrations of SMPs to detect the protective effects of SMPs on CCL4-induced hepatocyte injury. The results of the control group showed that chicken hepatocytes grew well and their morphology was normal. After CCL4 treatment, the activity of alanine transaminase (ALT) and aspartate transaminase (AST) of hepatocytes increased compared with the normal control group. SMPs treatment downregulated the contents of ALT, AST, and malondialdehyde (MDA), and upregulated the contents of glutathione (GSH) and cytochrome P450 (CYP450). An acute chicken liver injury model was established in vivo with 2.0 mL/kg 50% CCL4. Oral administration of SMP at different doses exhibited preventive success. The results showed that compared with the control group, the contents of total protein (TP), albumin (Alb), and GSH in the liver injury model group were significantly decreased and the levels of liver index, ALT, AST, and MDA were significantly increased. In contrast, in the SMP group the contents of TP, Alb, and GSH were significantly increased, and the levels of liver index, ALT, AST, and MDA were significantly decreased compared with the model group. Therefore, we conclude that SMPs have good protective effect on chicken liver damage in vivo and in vitro.
机译：本研究研究了丹参米尔蒂氏菌多糖（SMPS）对体外和体内鸡肝损伤的影响。在体外研究中，通过2步胶原酶灌注分离原发性培养的肝细胞。向肝细胞中加入四氯化碳（CCl4）以建立肝细胞损伤模型。用不同浓度的SMP处理肝细胞，以检测SMPS对CCL4诱导的肝细胞损伤的保护作用。对照组的结果表明，鸡肝肝细胞很好，它们的形态正常。在CCL4处理后，与正常对照组相比，肝细胞的丙氨酸转氨酶（ALT）和天冬氨酸转氨酶（AST）的活性增加。 SMPS治疗下调了ALT，AST和丙二醛（MDA）的含量，并上调谷胱甘肽（GSH）和细胞色素P450的含量（CYP450）。体内建立急性鸡肝损伤模型，其中2.0ml / kg 50％CCl4。口服SMP在不同剂量上表现出预防成功。结果表明，与对照组相比，肝损伤模型组中总蛋白质（TP），白蛋白（ALB）和GSH的含量明显下降，肝脏指数，ALT，AST和MDA的水平显着下降增加。相反，在SMP组中，与模型组相比，TP，ALB和GSH的内容显着增加，肝脏指数，ALT，AST和MDA的水平显着降低。因此，我们得出结论，SMP对体内和体外鸡肝损伤具有良好的保护作用。

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