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首页> 外文期刊>Plasmid: An International Journal Devoted to Extrachromosomal Gene Systems >Conjugation efficiency depends on intra and intercellular interactions between distinct plasmids: Plasmids promote the immigration of other plasmids but repress co-colonizing plasmids
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Conjugation efficiency depends on intra and intercellular interactions between distinct plasmids: Plasmids promote the immigration of other plasmids but repress co-colonizing plasmids

机译：共轭效率取决于不同质粒之间的内外相互作用：质粒促进其他质粒的移民，但抑制共殖民质粒

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Abstract Conjugative plasmids encode the genes responsible for the synthesis of conjugative pili and plasmid transfer. Expression of the conjugative machinery (including conjugative pili) may be costly to bacteria, not only due to the energetic/metabolic cost associated with their expression but also because they serve as receptors for certain viruses. Consequently, the presence of two plasmids in the same cell may be disadvantageous to each plasmid, because they may impose a higher fitness cost on the host. Therefore, plasmids may encode mechanisms to cope with co-resident plasmids. Moreover, it is possible that the transfer rate of a plasmid is affected by the presence of a distinct plasmid in the recipient cell. In this work, we measured transfer rates of twelve natural plasmids belonging to seven incompatibility groups in three situations, namely when: (i) donor cells contain a plasmid and recipient cells are plasmid-free; (ii) donor cells contain two unrelated plasmids and recipient cells are plasmid-free; and (iii) half of the cells contain a given plasmid and the other half contain another, unrelated, plasmid. In the third situation, recipient cells of a plasmid are the donor cells of the other plasmid. We show that there are more negative interactions (reduction of a plasmid's conjugative efficiency) between plasmids if they reside in the same cell than if they reside in different cells. However, if plasmids interacted intercellularly, the transfer rate of one of the plasmids was often higher (when the unrelated conjugative plasmid was present in the recipient cell) than if the recipient cell was plasmid-free – a positive effect. Experimental data retrieved from the study of mutant plasmids not expressing conjugative pili on the cell surface suggest that positive effects result from a higher efficiency of mating pair formation. Overall, our results suggest that negative interactions are significantly more frequent when plasmids occupy the same cell. Such interactions may determine how antibiotic resistance disseminates in bacterial populations. Highlights ? Conjugative plasmids influence each other's transfer rate. ? A plasmid is more prone to reduce conjugative efficiency of a co-resident plasmid than of a plasmid present at another cell. ? The transfer rate of a conjugative plasmid often increases if a distinct plasmid is present at the recipient cell.
机译：摘要缀合质粒编码负责合成共轭菌毛和质粒转移的基因。结合机械（包括缀合物Pili）的表达可能对细菌的成本高，而不仅仅是由于与其表达相关的能量/代谢成本，而且因为它们作为某些病毒的受体。因此，对同一细胞中两个质粒的存在可能对每个质粒不利，因为它们可能对宿主施加较高的健康成本。因此，质粒可以编码以应对共同植物质粒的机制。此外，质粒的转移速率可能受到受体细胞中存在不同质粒的影响。在这项工作中，我们在三种情况下测量了属于七种不相容性基团的12个天然质粒的转移率，即：（i）供体细胞含有质粒，可使受体细胞免受粒子体; （ii）供体细胞含有两个不相关的质粒，并且受体细胞是无细胞的; （iii）细胞中的一半含有给定的质粒，另一半含有另一种不相关的质粒。在第三种情况下，质粒的受体细胞是其他质粒的供体细胞。我们表明，如果它们位于相同的细胞中，则在质粒之间存在更大的相互作用（降低质粒的缀合效率），如果它们位于不同的细胞中。然而，如果质粒间细胞间相互作用，则其中一种质粒的转移率通常更高（当在受体细胞中存在不相关的缀合质粒时），而不是如果受体细胞没有质粒 - 阳性作用。从细胞表面上不表达缀合物的突变质粒的研究检出的实验数据表明，阳性效应是由较高的配合对形成的效率产生。总体而言，我们的结果表明，当质粒占据相同的细胞时，负相互作用明显更频繁。这种相互作用可以确定如何在细菌群体中传播抗生素抗性。强调 ？共轭质粒影响彼此的转移率。还更容易发生质粒，以减少共同驻留质粒的共轭效率，而不是另一种细胞中存在的质粒。还如果在受体细胞处存在不同的质粒，则缀合质粒的转移率通常会增加。

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