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首页> 外文期刊>Preparative biochemistry & biotechnology: An international journal for rapid communication >Chitosan modified Fe3O4/graphene oxide nanocomposite as a support for high yield and stable immobilization of cellulase: its application in the saccharification of microcrystalline cellulose
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Chitosan modified Fe3O4/graphene oxide nanocomposite as a support for high yield and stable immobilization of cellulase: its application in the saccharification of microcrystalline cellulose

机译：壳聚糖改性Fe3O4 /石墨烯氧化物纳米复合材料作为纤维素酶的高产量和稳定固定的载体：其在微晶纤维素的糖化中的应用

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摘要

In the present study, specialized methodology of utilizing novel nanobiocatalyst, chitosan coated magnetic graphene (Fe3O4/GO/CS), for efficacious immobilization of Trichoderma reesei cellulase, an important industrial enzyme was revealed. The cellulase was covalently immobilized onto the nanocomposite (NC) using covalent-glutaraldehyde coupling methodology. Successful immobilization of the cellulase with Fe3O4/GO/CS NC was affirmed by transmission electron microscopy, Fourier transform infrared spectroscopy and scanning electron microscopy. The nanobiocatalyst preparations exhibited significantly improved activity, retaining 78% of the initial activity as compared to its soluble counterpart. Immobilization of cellulase also highlighted significant broadening in pH, thermal and storage stability profiles. The kinetic properties of cellulase bound Fe3O4/GO/CS NC showed lower K-m indicating increased affinity (1.87 times) of nanobioconjugate toward the substrate. Cellulase bound Fe3O4/GO/CS NC was able to maintain higher percentages of its primary activity after 8 repeated uses. This nanobioconjugate preparation proves to be industrially robust biocatalyst with enhanced nano-biocatalytic activity, stability and reusability attributes, which can be exploited for hydrolysis of microcrystalline cellulose providing increased amount of sugar compared to free cellulase.
机译：在本研究中，利用新型纳米双催化剂的专用方法，壳聚糖涂层磁性石墨烯（Fe3O4 / Go / Cs），揭示了一种重要的工业酶的ricrocerma reesei纤维素酶的有效固定。使用共价 - 戊二醛偶联方法将纤维素酶共价固定到纳米复合物（NC）上。通过透射电子显微镜，傅里叶变换红外光谱和扫描电子显微镜肯定了纤维素酶的成功固定用Fe 3 O 4 / Go / CS NC。纳米双催化剂制剂表现出显着改善的活性，与其可溶性对应相比，保留初始活性的78％。纤维素酶的固定化也强调了pH，热和储存稳定性型材的显着扩展。纤维素酶结合Fe3O4 / Go / CS NC的动力学性质显示出低于K-M表示朝向基材的纳米芯缀缀合物的增加（1.87倍）。纤维素酶结合Fe3O4 / Go / CS NC能够在8次重复用途后保持较高百分比其初级活性。该纳米核缀合物制剂证明是具有增强的纳米生物催化活性，稳定性和可重复使用性的工业上稳健的生物催化剂，其可以利用与游离纤维素酶相比，用于提供增加的糖量的微晶纤维素的水解。

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