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首页> 外文期刊>Progress in Artificial Intelligence >Translocation of Drought-Responsive Proteins from the Chloroplasts
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Translocation of Drought-Responsive Proteins from the Chloroplasts

机译：从叶绿体中易腐蚀蛋白的易位

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Some chloroplast proteins are known to serve as messengers to transmit retrograde signals from chloroplasts to the nuclei in response to environmental stresses. However, whether particular chloroplast proteins respond to drought stress and serve as messengers for retrograde signal transduction are unclear. Here, we used isobaric tags for relative and absolute quantitation (iTRAQ) to monitor the proteomic changes in tobacco (Nicotiana benthamiana) treated with drought stress/re-watering. We identified 3936 and 1087 differentially accumulated total leaf and chloroplast proteins, respectively, which were grouped into 16 categories. Among these, one particular category of proteins, that includes carbonic anhydrase 1 (CA1), exhibited a great decline in chloroplasts, but a remarkable increase in leaves under drought stress. The subcellular localizations of CA1 proteins from moss (Physcomitrella patens), Arabidopsis thaliana and rice (Oryza sativa) in P. patens protoplasts consistently showed that CA1 proteins gradually diminished within chloroplasts but increasingly accumulated in the cytosol under osmotic stress treatment, suggesting that they could be translocated from chloroplasts to the cytosol and act as a signal messenger from the chloroplast. Our results thus highlight the potential importance of chloroplast proteins in retrograde signaling pathways and provide a set of candidate proteins for further research.
机译：已知一些叶绿体蛋白可以作为使者将逆行信号从叶绿体传递给核的逆行信号，以应对环境应力。然而，特定叶绿体蛋白是否应对干旱胁迫并用作逆行信号转导的信使尚不清楚。在这里，我们使用了用于亲属和绝对定量（ITRAQ）的等因素标签，以监测用干旱胁迫/再浇水处理的烟草（尼古利亚岛果儿）的蛋白质组学变化。我们鉴定了3936和1087型差异累积的总叶和叶绿体蛋白，分为16类。其中，一种特定类别的蛋白质，包括碳酸酐酶1（Ca1），在叶绿体中表现出很大的下降，但在干旱胁迫下叶片显着增加。来自苔藓（Physcomatralla Patens），拟南芥和米饭（Oryza Sativa）的Ca1蛋白的亚细胞定位始终显示Ca1蛋白在叶绿体中逐渐减少，但在渗透胁迫处理下越来越积聚，表明它们可以将从叶绿体转移到胞粒骨中并用作来自叶绿体的信号信使。因此，我们的结果突出了叶绿体蛋白在逆行信号传导途径中的潜在重要性，并提供了一组候选蛋白质进​​行进一步研究。

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