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首页> 外文期刊>Progress in Artificial Intelligence >Accuracy of the QIAxcel Automated System for MIRU-VNTR Genotyping of Mycobacterium tuberculosis in Two Limited Resource Settings
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Accuracy of the QIAxcel Automated System for MIRU-VNTR Genotyping of Mycobacterium tuberculosis in Two Limited Resource Settings

机译：两次有限资源设置中麦鲁-VNTR基因分型麦鲁-VNTR基因分型的准确性

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摘要

Mycobacterial interspersed repetitive units variable number tandem repeat (MIRU-VNTR) typing of Mycobacterium tuberculosis complex (MTBC) isolates, based on 24 loci, is still widely used as the standard for routine molecular surveillance of tuberculosis (TB). QIAxcel system is proposed as an affordable tool that could replace conventional gel electrophoresis and provide high concordance with the reference methods regarding MIRU-VNTR typing of MTBC. We aimed to evaluate the QIAxcel accuracy for allele calling of MIRU-VNTR loci in two regional reference laboratories. A total of 173 DNA were used for the study. Results obtained with QIAxcel were compared to the reference results obtained with an ABI 3730 DNA analyzer. In Albania, the overall agreement with the reference method was 97.92%. A complete agreement result was obtained for 17 loci. In Tunisia, the overall agreement with the reference method was 98.95%. A complete agreement result was obtained for 17 loci. Overall agreement in both centers was 98.43%. In our opinion, use of QIAxcel technology has the potential to be reliable, given an optimized algorithm. Inaccuracies in sizing of long fragments should be solved, especially regarding locus 4052.
机译：基于24个基因杆菌的分枝杆菌结核复合物（MTBC）分离物的分枝杆菌交叉重复单位可变数量串联重复（MIRU-VNTR）分类仍然被广泛地用作结核病（TB）的常规分子监测标准。 QIAXCEL系统被提出为可负担得起的工具，可以取代常规凝胶电泳，并通过关于MIRU-VNTR键入MTBC的参考方法提供高度一致。我们旨在评估两个区域参考实验室中Miru-VNTR基因座等位基因呼叫的QIAxcel准确性。共有173个DNA用于该研究。将用QIABEC获得的结果与ABI 3730DNA分析仪获得的参考结果进行比较。在阿尔巴尼亚，与参考方法的总体协议为97.92％。 17个基因座获得完整的协议结果。在突尼斯，与参考方法的总体协议为98.95％。 17个基因座获得完整的协议结果。两个中心的总体协议为98.43％。在我们看来，考虑到优化算法，使用Qiaxcel技术的使用具有可靠的可能性。应解决长碎片尺寸的不准确性，特别是关于基因座4052。

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