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Profiling of Flavonoid and Antioxidant Activity of Fruit Tissues from 27 Chinese Local Citrus Cultivars

机译:来自27例中国局部柑橘品种水果组织的类黄酮和抗氧化活性的分析

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Flavonoid profile and antioxidant activity of citrus peels, pulps, and juices from 27 local citrus cultivars in China were investigated. Flavonoid composition and content were determined using UPLC-PDA. Total phenolic content (TPC) and total flavonoid content (TFC) were measured using a Folin-Ciocalteau reagent and Al(NO3)-NaNO2 complexometry, respectively. The antioxidant capacities of the extracts were evaluated by DPPH, ABTS and FRAP method, respectively. Citrus peel not only exhibited better antioxidant potential, but also presented more composition diversity and contained higher concentrations of flavonoids than pulp and juice. Different citrus species were characterized by their individual predominant flavonoids, contributing largely to the antioxidant activity, such as mandarin was characterized by hesperidin, nobiletin and tangeretin, while pummelo and papeda were characterized by naringin. The peel of Guihuadinanfeng (Citrus reticulata) had the highest TPC of 23.46 mg equivalent gallic acid/g DW (dry weight) and TFC of 21.37 mg equivalent rutin/g DW. Shiyueju (C. reticulata) peel showed the highest antioxidant capacity based on the antioxidant potency composite (APC) analysis. Overall, mandarin (C. reticulata) fruits peel contained more TPC and TFC, exhibiting higher antioxidant capacities than other species, and were good natural sources of flavonoids and antioxidants.
机译:研究了中国柑橘皮,纸浆和中国局部柑橘品种的柑橘皮,纸浆和果汁的黄酮类化型和抗氧化活性。使用UPLC-PDA测定类黄酮组成和含量。使用Folin-Ciocalteau试剂和Al(NO 3) - NANO2复杂度分别测量总酚醛含量(TPC)和总类黄酮含量(TFC)。通过DPPH,ABTS和FRAP方法评估提取物的抗氧化能力。柑橘剥离不仅表现出更好的抗氧化潜力,而且还呈现了更多的组成多样性,并且含有比纸浆和果汁更高浓度的黄酮类化合物。不同的柑橘物种的特征在于它们的个体主要类黄酮,很大程度上促进抗氧化活性,例如普通蛋白,Nobiletin和橘子的特征在于普通蛋白,而Pummelo和Papeda的特征在于Naringin。桂花甘蓝果(柑橘类网状)的果皮具有23.46mg等当量的TPC的最高TPC(干重)和21.37mg当量rutin / g dw的TFC。 Shiyueju(C. Reticulata)Peel展示了基于抗氧化效力复合材料(APC)分析的最高抗氧化能力。总体而言,普通话(C. Reticulata)果实剥皮含有更多TPC和TFC,表现出比其他物种更高的抗氧化能力,并且是黄酮类化合物和抗氧化剂的良好天然来源。

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