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High Nitrogen Enhance Drought Tolerance in Cotton through Antioxidant Enzymatic Activities, Nitrogen Metabolism and Osmotic Adjustment

机译:通过抗氧化剂酶活性,氮代谢和渗透调节,高氮增强棉花的耐旱性

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Drought is one of the most important abiotic stresses and hampers many plant physiological processes under suboptimal nitrogen (N) concentration. Seedling tolerance to drought stress is very important for optimum growth and development, however, the enhancement of plant stress tolerance through N application in cotton is not fully understood. Therefore, this study investigates the role of high N concentration in enhancing drought stress tolerance in cotton. A hydroponic experiment supplying low (0.25 mM) and high (5 mM) N concentrations, followed by 150 g L-1 polyethylene glycol (PEG)-induced stress was conducted in a growth chamber. PEG-induced drought stress inhibited seedling growth, led to oxidative stress from excessive malondialdehyde (MDA) generation, and reduced N metabolism. High N concentrations alleviated oxidative damage and stomatal limitation by increasing antioxidant enzymatic activities, leaf relative water content, and photosynthesis in cotton seedlings under drought stress. The results revealed that the ameliorative effects of high N concentration may be ascribed to the enhancement of N metabolizing enzymes and an increase in the amounts of osmoprotectants like free amino acids and total soluble protein. The present data suggest that relatively high N concentrations may contribute to drought stress tolerance in cotton through N metabolism, antioxidant capacity, and osmotic adjustment.
机译:干旱是最重要的非生物应激之一,并且在次优氮(N)浓度下许多植物生理过程中的许多植物生理过程。对干旱胁迫的幼苗耐受性对于最佳的生长和发展非常重要,然而,通过N棉花施加的植物胁迫耐受性的增强尚不完全理解。因此,本研究调查了高N浓度在提高棉花中的干旱胁迫耐受性方面的作用。提供低(0.25mm)和高(5mm)n浓度的水培实验,然后在生长室中进行150g L-1聚乙二醇(PEG)诱导的应力。 PEG诱导的干旱胁迫抑制幼苗生长,导致来自过量丙二醛(MDA)产生的氧化胁迫,并降低N代谢。通过在干旱胁迫下增加棉花幼苗中的抗氧化酶活性,叶相对含水量和光合作用,高N浓度减轻氧化损伤和气孔限制。结果表明,高N浓度的改进效应可以归因于N代谢酶的增强和增加的渗透氨基酸和总可溶性蛋白质的量增加。本数据表明,相对较高的N浓度可能有助于通过N代谢,抗氧化能力和渗透调节棉花胁迫耐受性。

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