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首页> 外文期刊>Postharvest Biology and Technology >Insights into the changes in chemical compositions of the cell wall of pear fruit infected by Alternaria alternata with confocal Raman microspectroscopy
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Insights into the changes in chemical compositions of the cell wall of pear fruit infected by Alternaria alternata with confocal Raman microspectroscopy

机译：Concocal Raman Micropectopopy感染的梨果细胞壁细胞壁化学成分的变化见解

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摘要

In this paper, fresh pear fruits and those infected by Alternaria alternate (A. alternate) were studied by confocal Raman microspectroscopy (CRM) to illustrate the changes in chemical compositions of cell wall. Firstly, Raman spectra of the cell wall of both fresh and infected fruits were collected with spatial resolution at micron level, and then label-free in situ imaging of chemical compositions in the cell wall were mapped. The results showed that there were significant changes in the signal intensity of cell wall, especially in the later stage of A. alternate infection. After 8 days of infection at room temperature, the signal intensities at 1086 and 871 cm(-1), which were associated with cellulose and pectin, were decreased by 58.50% and 58.67%, respectively, revealing changes in the main components of the infected cell wall infected. Meanwhile, the chemical images of the cell wall of both fresh and infected fruits were compared, indicating that A. alternate infection caused the alterations of morphological structure and chemical compositions in the cell wall in a time-dependent manner. Our results confirmed that CRM is a useful tool for the identification of compositional changes in the cell wall caused by fungal infection without the need for any chemical treatment. For the first time, the current research applied CRM in phytopathology for investigating interactive relationship between a pathogen and its host, thus offering a new way for in-depth study of pathogen-host interactions at cellular level.
机译：在本文中，通过共焦拉曼微穴位（CRM）研究了新鲜梨果和由alterararia交替（A.替代）的那些。首先，将新鲜和感染的水果的细胞壁的拉曼光谱与微米水平的空间分辨率收集，然后在细胞壁中的化学组合物中没有标记的映射。结果表明，细胞壁的信号强度有显着变化，特别是在A.替代感染的后期阶段。在室温下感染8天后，1086和871cm（-1）的信号强度分别与纤维素和果胶相关联，分别下降58.50％和58.67％，揭示了受感染的主要成分的变化感染细胞壁。同时，比较了新鲜和受感染的水果的细胞壁的化学图像，表明A.替代感染使得在细胞壁中以时间依赖性方式改变形态结构和化学组成的改变。我们的结果证实，CRM是一种有用的工具，用于鉴定由真菌感染引起的细胞壁中的组成变化，而无需任何化学处理。首次，目前的研究应用CRM在植物病理学中用于研究病原体和宿主之间的互动关系，从而为细胞水平进行了深入研究病原体宿主相互作用的新方法。

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