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首页> 外文期刊>Pulmonary pharmacology & therapeutics >Asthma phenotypes and T-bet protein expression in cells treated with Fluticasone Furoate/Vilanterol
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Asthma phenotypes and T-bet protein expression in cells treated with Fluticasone Furoate/Vilanterol

机译：用氟辛酸甲酯/类化合物处理的细胞中的哮喘表型和T-BET蛋白表达

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摘要

Asthma is a complex disease with diverse clinical manifestations ranging from mild to severe. Despite existing guidelines for asthma recognition and treatment, still a proportion of patients stay uncontrolled. Combinational therapy which comprises inhaled corticosteroids (ICS) and a long acting B2 adrenreceptor agonist (LABA) has been suggested to control asthma. In this study T-bet expression was attested in CD4 T cells treated with Fluticasone Furoate (FF), Vilanterol (V) and FF/V combination in severe asthmatic patients compared to patients with moderate asthma and healthy controls using Immunocytochemistry (ICC). First, CD4 T cells were isolated from PBMCs of 12 patients and controls using CD4 T cell isolation kit. Subsequently, isolated CD4 T cells were cultured with FF, V and FF/V for 1 h. To accomplish ICC, cells were incubated with anti-T-bet antibody, and then stained with HRP-bound secondary antibody. T-bet expression was evaluated using light microscopy. Statistical analyses were performed using R 3.5.2 software and visualized by ggplot2 3.1.0 package. Significant increasing in T-bet expression was seen in CD4 T cells from patients with moderate asthma treated with FF and FF/V. Suggesting conclusion would be distinct mechanisms responsible for severe asthma and moderate asthma in the patients and the needs for novel therapies. Further molecular studies in different asthma phenotypes would be instructive for asthma treatment.
机译：哮喘是一种复杂的疾病，具有不同于轻度至严重的临床表现。尽管现有的哮喘识别和治疗准则，但仍然是患者的比例不受控制。组合疗法包括吸入的皮质类固醇（IC）和长作用B2肾上腺诱导激动剂（Laba）对抗哮喘。在该研究中，在使用免疫细胞化学（ICC）的中度哮喘和健康对照的患者中，在用氟酮呋磺酸盐（FF），Vilantol和FF / V组合处理的CD4 T细胞中，在CD4 T细胞中证明T-BET表达。首先，使用CD4 T细胞分离试剂盒从12名患者的PBMC分离CD4 T细胞。随后，用FF，V和FF / V培养分离的CD4 T细胞1小时。为了完成ICC，将细胞与抗T-BET抗体一起温育，然后用HRP结合的二抗染色。使用光学显微镜评估T-BET表达。使用R 3.5.2软件进行统计分析，并通过GGPLOT2 3.1.0包进行可视化。从使用FF和FF / v的中度哮喘患者的CD4 T细胞中看到T-BET表达的显着增加。建议结论是对患者中严重哮喘和中度哮喘的不同机制以及新疗法的需求。对不同哮喘表型的进一步分子研究对于哮喘治疗是有效的。

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