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首页> 外文期刊>Quaternary geochronology >Protein diagenesis in archaeological shells of Littorina littorea (Linnaeus, 1758) and the suitability of this material for amino acid racemisation dating
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Protein diagenesis in archaeological shells of Littorina littorea (Linnaeus, 1758) and the suitability of this material for amino acid racemisation dating

机译：Littorina Littorea（Linnaeus，1758）的考古壳中的蛋白质成岩作用以及这种材料的适用性氨基酸脱模约会

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摘要

The inter- and intra-crystalline fractions of Littorina littorea periwinkles recovered from archaeological sites in Northern Spain (covering the Azilian, Magdalenian and Solutrean periods) were examined for amino acid composition and racemisation over time. These fractions were found to be composed of similar proteins, as the amino acids and Asx D/L values were comparable and varied in the same way with increasing age and heating time. The protein loss from the inter-crystalline fraction was not highly significant after 22 ka and was lower than in other archaeological mollusc shells (Phorcus lineatus and Patella vulgata). The intra-crystalline fraction, isolated by bleaching, appeared to behave as a closed system for at least 22 ka, as reflected by the lack of a significant decrease in the amino acid content, although changes in the amino acid percentages occurred during this period. The percentage of aspartic acid remained almost constant with age both in the inter- and intra-crystalline fractions. Good correspondence was obtained between Asx D/L values in unbleached and bleached samples and age, but discrimination of these archaeological periods within the last 27 ka using amino acid racemisation of L. littorea is unclear due to only slight differences between D/L values. We propose that temperature played a key role in the amino acid racemisation of L. littorea and could explain why in the localities that were formed during relatively cold conditions during the Lower Magdalenian (20.5-16.3 ka cal BP) and Solutrean (ca. 26.5-20.5 ka cal BP) periods, D/L values were similar and only slightly higher than in shells from sites formed during the Upper Magdalenian (16.3-13.5 ka cal BP).
机译：在西班牙北部的考古位点（覆盖亚丁利亚，Magdalenian和SoluteRean）的考古位点中回收的肝脏内部分数被检查为氨基酸组成和随时间的求导。发现这些级分由类似的蛋白质组成，因为氨基酸和ASX D / L值与随着年龄和加热时间的增加，以相同的方式变化。在22ka后，结晶间馏分的蛋白质损失在其他考古莫尔斯康壳（Phorcus Lineatus和Patellaulgata）中不太显着。通过漂白分离的结晶级分，似乎表现为至少22ka的封闭系统，因为缺乏氨基酸含量的显着降低反映，尽管在此期间发生氨基酸百分比的变化。在结晶间级分中，天冬氨酸的百分比几乎持续。在未漂白的和漂白样品和漂白样品中的ASX D / L值之间获得了良好的对应，但是使用L. Littorea的氨基酸术语在过去27ka内的这些考古时期的判断尚不清楚D / L值之间的微小差异。我们提出这种温度在L. Littorea的氨基酸出色中发挥了关键作用，并且可以解释为什么在较低的Magdalenian（20.5-16.3 Ka Cal BP）和Solutrean（约26.5- 20.5 ka Cal BP）期间，D / L值相似，仅略高于上槌中所形成的位点的壳（16.3-13.5 ka Cal BP）。

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