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首页> 外文期刊>Pathology oncology research: POR >Correlations of IGF-1R and COX-2 Expressions with Ras and BRAF Genetic Mutations, Clinicopathological Features and Prognosis of Colorectal Cancer Patients
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Correlations of IGF-1R and COX-2 Expressions with Ras and BRAF Genetic Mutations, Clinicopathological Features and Prognosis of Colorectal Cancer Patients

机译：IGF-1R和COX-2表达与RAS和BRAF基因突变的相关性，结直肠癌患者的临床病理特征和预后

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Abstract This case-control study aims to investigate the correlations of insulin-like growth factor receptor 1 (IGF-1R) and cyclooxygenase 2 (COX-2) expressions with Ras and BRAF genetic mutations, clinicopathological features and prognosis of colorectal cancer (CRC) patients. A total of 213 CRC patients (case group) and 200 healthy individuals (control group) were selected from our hospital. Ras (K-Ras/N-Ras) and BRAF genetic mutations were detected by direct sequencing. The positive expression rates of IGF-IR and COX-2 in CRC and normal tissues were detected using immunohistochemistry. RT-qPCR and Western blotting were applied to detect the mRNA and protein expressions of IGF-IR and COX-2 in CRC tissues and normal tissues. Total mutation rate of N-Ras , BRAF and K-Ras in case group were 5.2%, 12.2% and 47.4%, respectively. The expressions of IGF-IR and COX-2 were higher in CRC tissues with Ras and BRAF mutations than in those without. CRC tissues with Ras mutation showed higher COX-2 expression than those with BRAF mutation. IGF-IR and COX-2 expressions were correlated to infiltration degree, lymphatic metastasis (in CRC tissues with and without Ras and BRAF mutations), and Dukes stages (only in CRC tissues with Ras and BRAF mutations). CRC patients with negative expressions of IGF-IR and COX-2 had significantly higher accumulative survival rate and longer mean survival duration than those with positive expressions of IGF-IR and COX-2. These findings indicate that IGF-1R and COX-2 expressions may be associated with Ras and BRAF genetic mutations, clinicopathological feature and prognosis of CRC patients.
机译：摘要本病例对照研究旨在探讨胰岛素样生长因子受体1（IGF-1R）和环氧化酶2（COX-2）表达式与RAS和BRAF遗传突变，临床病理特征和结肠直肠癌（CRC）的预后的相关性的相关性耐心。我们医院中共有213名CRC患者（病例组）和200名健康个体（对照组）。通过直接测序检测Ras（K-RAS / N-RAS）和BRAF基因突变。使用免疫组织化学检测CRC和正常组织中IGF-IR和COX-2的阳性表达速率。施用RT-QPCR和Western印迹以检测CRC组织和正常组织中IGF-IR和COX-2的mRNA和蛋白表达。壳体组中N-RAS，BRAF和K-RA的总突变率分别为5.2％，12.2％和47.4％。 IGF-IR和COX-2的表达在CRC组织中较高，RAS和BRAF突变比在没有的情况下。具有RAS突变的CRC组织显示比具有BRAF突变的COX-2表达更高。 IGF-IR和COX-2表达与浸润程度相关，淋巴结转移（在具有和没有RAS和BRAF突变的CRC组织中）和Dukes阶段（仅在RAS和BRAF突变中的CRC组织中）。 CRC患有IGF-IR和COX-2的阴性表达的患者具有显着较高的累积存活率和比IGF-IR和COX-2的阳性表达的持续时间更高。这些发现表明IGF-1R和COX-2表达可以与RAS和BRAF遗传突变，CRC患者的临床病理特征和预后相关。

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