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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Characterizing Reactivity to Onchocerca volvulus Antigens in Multiplex Bead Assays
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Characterizing Reactivity to Onchocerca volvulus Antigens in Multiplex Bead Assays

机译：特征在多重珠子测定中对Onchocerca Volvulus抗原的反应性

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摘要

Multiplex bead assays (MBAs) may provide a powerful integrated tool for monitoring, evaluation, and post-elimination surveillance of onchocerciasis and co-endemic diseases; however, the specificity and sensitivity of Onchocerca volvulus antigens have not been characterized within this context. An MBA was developed to evaluate three antigens (OV-16, OV-17, and OV-33) for onchocerciasis. Receiver operating characteristics (ROC) analyses were used to characterize antigen performance using a panel of 610 specimens: 109 O. volvulus-positive specimens, 426 non-onchocerciasis controls with filarial and other confirmed parasitic infection, and 75 sera from patients with no other parasitic infection. The IgG and IgG4 assays for OV-16 demonstrated sensitivities of 95.4% and 96.3%, and specificities of 99.4% and 99.8%, respectively. The OV-17 IgG and IgG4 assays had sensitivities of 86.2% and 76.1% and specificities of 79.2% and 82.8%. For OV-33, the IgG and IgG4 assays had sensitivities of 90.8% and 96.3%, and specificities of 96.8% and 98.6%. The OV-16 IgG4-based MBA had the best assay characteristics, followed by OV-33 IgG4. The OV-16 IgG4 assay would be useful for monitoring and evaluation using the MBA platform. Further evaluations are needed to review the potential use of OV-33 as a confirmatory test in the context of program evaluations.
机译：多重珠子测定（MBA）可以提供强大的综合工具，用于监测，评估和消除onchocerciaSis和合作疾病的监测;然而，在这种情况下尚未表征onchocerca抗原的特异性和敏感性。开发了MBA以评估三种抗原（OV-16，OV-17和OV-33）以进行onchocercivesis。接收器操作特性（ROC）分析用于使用610个标本面板表征抗原性能：109 o. Volvulus阳性标本，426个非甘露病毒对照，与丝状和其他确诊的寄生虫感染，75例血清没有其他寄生虫感染。 OV-16的IgG和IgG4测定分别显示出95.4％和96.3％的敏感性，并且分别为99.4％和99.8％的特异性。 OV-17 IgG和IgG4测定的敏感性为86.2％和76.1％，特异性为79.2％和82.8％。对于OV-33，IgG和IgG4测定的敏感性为90.8％和96.3％，特异性为96.8％和98.6％。基于OV-16 IgG4的MBA具有最佳的测定特性，其次是OV-33 IgG4。 OV-16 IgG4测定对于使用MBA平台进行监测和评估。需要进一步的评估来审查OV-33的潜在使用作为方案评估的背景下的确认测试。

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