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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >A Novel Assay for Simultaneous Assessment of Mammalian Host Blood, Mosquito Species, and Plasmodium spp. in the Medically Important Anopheles Mosquitoes of Madagascar
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A Novel Assay for Simultaneous Assessment of Mammalian Host Blood, Mosquito Species, and Plasmodium spp. in the Medically Important Anopheles Mosquitoes of Madagascar

机译：用于同时评估哺乳动物宿主血液，蚊子物种和疟原虫SPP的新型测定。 在Madagascar的医学上重要的anopheles蚊子

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摘要

Anopheles mosquitoes vary in habitat preference, feeding pattern, and susceptibility to various measures of vector control. Consequently, it is important that we identify reservoirs of disease, identify vectors, and characterize feeding patterns to effectively implement targeted control measures. Using 467 anopheline mosquito abdomen squashes captured in Madagascar, we designed a novel ligase detection reaction and fluorescent microsphere assay, dubbed Bloodmeal Detection Assay for Regional Transmission (BLOODART), to query the bloodmeal content, identify five Anopheles mosquito species, and detect Plasmodium infection. Validation of mammalian bloodspots was achieved by preparation and analysis of known hosts (singular and mixed), sensitivity to degradation and storage method were assessed through mosquito feeding experiments, and quantification was explored by altering ratios of two mammal hosts. BLOODART identifications were validated by comparison with mosquito samples identified by sequenced portions of the internal transcribed spacer 2. BLOODART identification of control mammal bloodspots was 100% concordant for singular and mixed mammalian blood. BLOODART was able to detect hosts up to 42 hours after digestion when mosquito samples were stored in ethanol. A mammalian host was identified in every field-collected, blood-fed female Anopheles mosquito by BLOODART. The predominant mosquito host was cow (n = 451), followed by pig (n = 26) and human (n = 25). Mixed species bloodmeals were commonly observed (n = 33). A BLOODART molecular identification was successful for 318/467 mosquitoes, with an overall concordance of 60% with all field-captured, morphologically identified Anopheles specimens. BLOODART enables characterization of large samples and simultaneous pathogen detection to monitor and incriminate disease vectors in Madagascar.
机译：蚊子蚊子在栖息地偏好，饲养模式和对各种措施对照措施的敏感性方面变化。因此，重要的是，我们识别疾病的储层，鉴定载体，以及表征饲养模式，以有效地实施目标控制措施。使用在马达加斯加捕获的467个激动线蚊帐南瓜，我们设计了一种新颖的连接酶检测反应和荧光微球测定，为区域传播（血迹）被称为血膜检测测定，查询血液含量，识别五种蚊虫物种，检测疟原虫感染。通过制备和分析已知宿主（单数和混合）来实现哺乳动物血栓度的验证，通过蚊子喂养实验评估对降解和储存方法的敏感性，通过改变两种哺乳动物宿主的比率来探讨定量。通过与由内部转录间隔物的测序部分鉴定的蚊子样本进行验证验证了血压鉴定。对照哺乳动物血液排放的流血鉴定是单数和混合哺乳动物血液的100％交响。当蚊子样品储存在乙醇中时，流血能够在消化后42小时检测到42小时。在每个野外收集的血液食用的女性粪便蚊子中被鉴定了哺乳动物宿主。主要的蚊子宿主是牛（n = 451），其次是猪（n = 26）和人（n = 25）。通常观察混合物种血液骨肉（n = 33）。血迹分子鉴定成功为318/467蚊子，整体一致性为60％，所有现场捕获的形态学鉴定的肌肉标本。血压使大型样品和同时病原体检测能够监测马达加斯加的疾病载体。

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