N160 of Aiolos Determines its DNA‐Binding Activity

Wang Hao; Xu Zhao; Du Wei; Lin Zhenzhen; Liu Zhe

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N160 of Aiolos Determines its DNA‐Binding Activity

机译：AIOLOS的N160决定了其DNA结合活性

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ABSTRACT Aiolos is a transcription factor of the Ikaros family. It is expressed in lymphocytes and plays a vital role in the development of lymphatic system. Recently, Aiolos has been found to be ectopically expressed in lung cancer cells and promotes cancer metastasis through repression of p66 Shc expression. Blocking DNA‐binding activity of Aiolos may benefit cancer patients by preventing further metastasis. However, it is not clear which sequence is essential for its DNA binding. In this study, we found that N160 is a key amino acid to determine the DNA‐binding activity of Aiolos. Mutation of N160A resulted in loss of peripheral heterochromatin localization and dissociation with its target gene. Accordingly, Aiolos with N160A could not change the expression of its target genes. Thus, we identified the amino acid (N160) that is essential for Aiolos binding to DNA. Anat Rec, 302:2014–2019, 2019. ? 2019 American Association for Anatomy

机译：摘要AIOLOS是ikaros家族的转录因子。它以淋巴细胞表达，并在淋巴系统的发育中起着至关重要的作用。最近，已发现AIOLOS在肺癌细胞中被异常表达，并通过抑制P66 SHC表达来促进癌症转移。阻断AIOLOS的DNA结合活性可以通过预防进一步转移来受益癌症患者。然而，目前尚不清楚哪种序列对于其DNA结合是必不可少的。在这项研究中，我们发现N160是确定AIOLOS的DNA结合活性的关键氨基酸。 N160a的突变导致外周异色蛋白定位和与其靶基因的解离。因此，具有N160A的AIOLOS不能改变其靶基因的表达。因此，我们确定了对DNA结合的AIOLOS是必不可少的氨基酸（N160）。 ANAT REC，302：2014-2019，2019年。 2019年美国解剖学协会

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《The anatomical record: advances in integrative anatomy and evolutionary biology》 |2019年第11期|共6页
作者
Wang Hao; Xu Zhao; Du Wei; Lin Zhenzhen; Liu Zhe;
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作者单位

Department of Immunology Tianjin Key Laboratory of Medical Epigenetics Collaborative Innovation;

Department of Immunology Tianjin Key Laboratory of Medical Epigenetics Collaborative Innovation;

Department of Immunology Tianjin Key Laboratory of Medical Epigenetics Collaborative Innovation;

Department of Immunology Tianjin Key Laboratory of Medical Epigenetics Collaborative Innovation;

Department of Immunology Tianjin Key Laboratory of Medical Epigenetics Collaborative Innovation;

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正文语种 eng
中图分类人体形态学;
关键词
Aiolos; N160; DNA binding; transcriptional regulation;

机译：AIOLOS;N160;DNA结合;转录调节;

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1. N160 of Aiolos Determines its DNA‐Binding Activity [J] . Wang Hao, Xu Zhao, Du Wei, The anatomical record: advances in integrative anatomy and evolutionary biology . 2019,第11期

机译：AIOLOS的N160决定了其DNA结合活性
2. Preliminary investigation of anticancer activity by determining the DNA binding and antioxidant potency of new ferrocene incorporated N,N0,N00-trisubstituted phenylguanidines [J] . Rukhsana Gul, Amin Badshah, Azim Khan, Spectrochimica acta, Part A. Molecular and biomolecular spectroscopy . 2014,第Null期

机译：通过确定新的二茂铁掺入的N，N0，N00-三取代的苯基胍的DNA结合力和抗氧化能力初步研究抗癌活性
3. DNA methylation and Sp1 binding determine the tissue-specific transcriptional activity of the mouse Abcc6 promoter. [J] . Douet V, Heller MB, Le-Saux O Biochemical and Biophysical Research Communications . 2007,第1期

机译：DNA甲基化和Sp1结合决定了小鼠Abcc6启动子的组织特异性转录活性。
4. Examining Flanking Sequence Specificity and Topological Specificity in the Binding of Various Molecular Types to DNAs Using Restriction Endonuclease Activity Assays [C] . S. A. Winkle, E. Duran, J. Pulido, Symposium on frontiers in nucleic acids . 2011

机译：使用限制性内切核酸酶活性测定检查各种分子类型与DNA的结合中的侧翼序列特异性和拓扑特异性
5. Transient kinetic studies on the mechanisms of DNA binding, DNA unwinding, and DNA-stimulated ATPase activities by the Escherichia coli Rep helicase. [D] . Hsieh, Chang-Tai John. 2002

机译：大肠杆菌Rep解旋酶对DNA结合，DNA释放和DNA刺激的ATPase活性机制的瞬态动力学研究。
6. Functional differences between the two splice variants of the nucleolar transcription factor UBF: the second HMG box determines specificity of DNA binding and transcriptional activity. [O] . A Kuhn, R Voit, V Stefanovsky, 1994

机译：核仁转录因子UBF的两个剪接变体之间的功能差异：第二个HMG盒确定DNA结合的特异性和转录活性。
7. Coupling of adenine nucleotide binding and hydrolysis to single- and double-stranded DNA binding determines the topoisomerase activity of reverse gyrase from "Thermotoga maritima" [O] . Jungblut Stefan Patrick 2009

机译：腺嘌呤核苷酸结合和水解与单链和双链DNa结合的偶联决定了来自“Thermotoga maritima”的反向旋转酶的拓扑异构酶活性。

N160 of Aiolos Determines its DNA‐Binding Activity

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