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首页> 外文期刊>The anatomical record: advances in integrative anatomy and evolutionary biology >Transplantation and Tracking of the Human Umbilical Cord Mesenchymal Stem Cell Labeled with Superparamagnetic Iron Oxide in Deaf Pigs
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Transplantation and Tracking of the Human Umbilical Cord Mesenchymal Stem Cell Labeled with Superparamagnetic Iron Oxide in Deaf Pigs

机译：聋猪超顺磁性氧化铁标记的人脐带间充质干细胞的移植及跟踪

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ABSTRACT The purpose of this study was to establish a safe and effective approach to label the human umbilical cord mesenchymal stem cells (UC‐MSCs) derived from the Wharton's Jelly with superparamagnetic iron oxide (SPIO) nanoparticles as a cell tracer. The cytotoxicity of the SPIO was screened in vitro by cytochemical experiments. The results showed the new infection protocol of SPIO‐Lip2000 mixture had high efficiency and the optimal labeling concentration was a 50?μg/ml SPIO suspension. Transmission electron microscope (TEM) confirmed the distribution of the intracellular SPIO. We transplanted the labeled UC‐MSCs into the sensorineural hearing loss (SNHL) minipigs at 1 week after noise exposure. Auditory brainstem response results demonstrated the transplantation of UC‐MSCs was an efficient therapy for SNHL. The positive sediments in cochlear blood vessels, the bony wall of scala tympani, and spiral ganglion nerve fibers were found in the stem cell recipients' cochlea. We did not detect iron elements in the inner/outer hair cells' stereocilia, cuticular plate, or pillar cells from the basal to apex turns of the stem cell recipients' cochlea. In addition, TEM found SPIO in the medulla oblongata and the cerebrum in the SNHL minipigs after stem cell transplantation. In conclusion, we established a safe and effective approach to labeled human UC‐MSCs derived from Wharton's Jelly by using SPIO nanoparticles as a cell tracer in vitro and in vivo . This protocol showed a wide promising application in stem cell therapy and tracing in vivo for experiments with large mammals. Anat Rec, 303:494–505, 2020. ? 2019 American Association for Anatomy
机译：摘要本研究的目的是建立一种安全有效的方法来标记从沃顿果冻的人的脐带间充质干细胞（UC-MSCs）与作为细胞示踪剂的超顺磁性氧化铁（SPIO）纳米颗粒。通过细胞化学实验，体外筛选硅藻菌毒性。结果表明，Spio-Lip2000混合物的新感染方案具有高效率，最佳标记浓度为50Ωμg/ ml硅藻悬浮液。透射电子显微镜（TEM）证实了细胞内硅藻的分布。在噪声暴露后1周将标记的UC-MSCs移植到感觉内听力损失（SNHL）MINIPIG中。听觉脑干响应结果表明，UC-MSCs的移植是SNHL的有效疗法。在干细胞接受者的耳蜗中发现了耳蜗血管，颅骨壁和螺旋神经节神经纤维的阳性沉积物。我们没有从基础上的基础上的内/外毛细胞的立体纤维菌，切割板或柱细胞中检测铁元素到干细胞受体的耳蜗的顶点转弯。此外，TEM在干细胞移植后SNHL MINIPIGS中的麦克麻椭圆形和脑中的校长。总之，我们通过在体外和体内使用硅藻纳米粒子作为细胞示踪剂，建立了一种安全有效的方法来标记从沃顿果冻衍生自沃顿果冻的人UC-MSCs。该方案表明，在干细胞疗法和追踪大型哺乳动物的实验中展示了广泛的有希望的应用。 ANAT REC，303：494-505,2020。 2019年美国解剖学协会

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