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首页> 外文期刊>The anatomical record: advances in integrative anatomy and evolutionary biology >Isolation and Characterization of Mammalian Otic Progenitor Cells that Can Differentiate into Both Sensory Epithelial and Neuronal Cell Lineages
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Isolation and Characterization of Mammalian Otic Progenitor Cells that Can Differentiate into Both Sensory Epithelial and Neuronal Cell Lineages

机译：哺乳动物耳祖细胞的分离和表征可以分化为感觉上皮和神经细胞谱系

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ABSTRACT The mammalian inner ear mediates hearing and balance and during development generates both cochleo‐vestibular ganglion neurons and sensory epithelial receptor cells, that is, hair cells and support cells. Cell marking experiments have shown that both hair cells and support cells can originate from a common progenitor. Here, we demonstrate the lineage potential of individual otic epithelial cell clones using three cell lines established by a combination of limiting dilution and gene‐marking techniques from an embryonic day 12 (E12) rat otocyst. Cell‐type specific marker analyses of these clonal lines under proliferation and differentiation culture conditions demonstrate that during differentiation immature cell markers (Nanog and Nestin) were downregulated and hair cell (Myosin VIIa and Math1), support cell (p27 Kip1 and cytokeratin) and neuronal cell (NF‐H and NeuroD) markers were upregulated. Our results suggest that the otic epithelium of the E12 mammalian inner ear possess multipotent progenitor cells able to generate cell types of both sensory epithelial and neural cell lineages when cultured under a differentiation culture condition. Understanding the molecular mechanisms of proliferation and differentiation of multipotent otic progenitor cells may provide insights that could contribute to the development of a novel cell therapy with a potential to initiate or stimulate the sensorineural repair of damaged inner ear sensory receptors. Anat Rec, 303:451–460, 2020. ? 2019 American Association for Anatomy
机译：摘要哺乳动物内耳介导听证和平衡以及在开发过程中产生科技室前庭神经节神经元和感觉上皮受体细胞，即毛细胞和支持细胞。细胞标记实验表明，毛细胞和支持细胞都可以源自常见的祖细胞。在这里，我们使用来自胚胎第12（E12）大鼠滴耳的稀释和基因标记技术的组合建立的三种细胞系来证明单个耳蜗细胞克隆的谱系潜力。细胞型特异性标记分析这些克隆物系在增殖和分化培养条件下表明，在分化期间，在分化的未成熟细胞标志物（纳米瘤和Nestin）期间是下调和毛细胞（肌球蛋白VIIA和MATH1），支持细胞（P27 KIP1和细胞角蛋白）和神经元上调细胞（NF-H和NEUROD）标记。我们的研究结果表明，E12哺乳动物内耳的耳蜗上皮具有能够在分化培养条件下培养时产生感觉上皮和神经细胞谱系的细胞类型的多能祖细胞。了解多能静脉祖细胞的增殖和分化的分子机制可以提供有助于开发新细胞疗法的洞察力，该潜力能够引发或刺激受损内耳感觉受体的感官修复。 ANAT REC，303：451-460,2020。 2019年美国解剖学协会

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