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首页> 外文期刊>The European Journal of Neuroscience >Optical analysis of functional development of the facial motor nucleus in the embryonic rat brainstem
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Optical analysis of functional development of the facial motor nucleus in the embryonic rat brainstem

机译：胚胎大鼠脑干面部电机核功能性发育的光学分析

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摘要

Abstract Facial motor neurons of the rat embryo are first generated in rhombomere 4 and then migrate in the caudoventral direction. This migration forms a unique axonal trajectory called the genu, a loop of facial motor axons around the abducens nucleus. It is still unclear when and how this unique structure is functionally established during ontogenesis. Using voltagesensitive dye (VSD) recording and the DiI staining method, we identified neural responses evoked by facial nerve (N.VII) stimulation and examined developmental processes of the facial motor nucleus in E12E17 rat brainstems. We identified two types of fast spikelike signals; a longduration signal, which corresponded to the action potential in the N.VII soma, and a shortduration signal, which reflected the action potential in the N.VII axons. The longduration signal was detected as early as E13, suggesting that the N.VII motor neuron is already excitable at the beginning of cell migration. The response area of the longduration signal extended caudally at E13E14, and shifted in a ventral direction at E15. At E16E17, the longduration signal was concentrated in the caudoventral area, which was comparable to the location of the facial motor nucleus in the adult rat brainstem. These results demonstrate that developmental processes of cell migration and nuclear organization can be visualized and identified functionally with the VSD recording. We discuss the results by comparing functiogenesis and morphogenesis of the N.VII pathway.
机译：摘要首先在菱形4中产生大鼠胚胎的面部电机神经元，然后在剖面方向上迁移。这种迁移形成了一个独特的轴突轨迹，称为nu，围绕abducens核的一个面部电机轴颈。尚不清楚在梭菌期间在功能上建立这种独特的结构何时以及如何确定。使用电压阻塞染料（VSD）记录和DII染色方法，我们确定了通过面神经（N.VII）刺激引起的神经反应，并检查了E1217大鼠脑干中面部电机核的发育过程。我们确定了两种类型的快速尖峰信号;漫长信号，其对应于N.VII SOMA中的动作电位和缩短信号，其反映了N.VII轴突中的动作电位。早在E13中检测到纵向信号，表明N.VII电动机神经元在细胞迁移开始时已经是激动的。纵向信号的响应区域在E13E14处透明地延伸，并在E15处以腹侧方向转移。在E16E17，延长信号浓缩在剖位区域中，其与成年大鼠脑干中面部电机细胞核的位置相当。这些结果表明，可以通过VSD录音来可视化和识别细胞迁移和核组织的发育过程。通过比较N.VII途径的血液发生和形态发生来讨论结果。

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