• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Subpopulations of vomeronasal sensory neurons with coordinated coexpression of type 2 vomeronasal receptor genes are differentially dependent on Vmn2r1
【24h】

Subpopulations of vomeronasal sensory neurons with coordinated coexpression of type 2 vomeronasal receptor genes are differentially dependent on Vmn2r1

机译：具有2型葡聚物受体基因的协调共表达的血管感觉神经元的亚族差异依赖于VMN2R1

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The mouse vomeronasal organ is specialized in the detection of pheromones. Vomeronasal sensory neurons (VSNs) express chemosensory receptors of two large gene repertoires, V1R and V2R, which encode G-protein-coupled receptors. Phylogeneti-cally, four families of V2R genes can be discerned as follows: A, B, C, and D. VSNs located in the basal layer of the vomeronasal epithelium coordinately coexpress V2R genes from two families: Approximately half of basal VSNs coexpress Vmn2r1 of family C with a single V2R gene of family A8-10, B, or D ('C1 type of V2Rs'), and the other half coexpress Vmn2r2 through Vmn2r7 of family C with a single V2R gene of family A1-6 ('C2 type V2Rs'). The regulatory mechanisms of the coordinated coexpression of V2Rs from two families remain poorly understood. Here, we have generated two mouse strains carrying a knockout mutation in Vmn2r1 by gene targeting in embryonic stem cells. These mutations cause a differential decrease in the numbers of VSNs expressing a given C1 type of V2R. There is no compensatory expression of Vmn2r2 through Vmn2r7. VSN axons coalesce into glomeruli in the appropriate region of the accessory olfactory bulb in the absence of Vmn2r1. Gene expression profiling by Nano-String reveals a differential and graded decrease in the expression levels across C1 type of V2Rs. There is no change in the expression levels of C2 type of V2Rs, with two exceptions that we reclassified as C1 type. Thus, there appears to be a fixed probability of gene choice for a given C2 type of V2R.
机译：小鼠群体器官专门用于检测信息素。呕吐物感觉神经元（VSNS）表达两种大型基因曲目，V1R和V2R的化学感受器，其编码G蛋白偶联受体。 phylogeneti-cly，4R基因的四个家族可以辨别如下：A，B，C和D. Vsns位于群体上皮的基底层中，与两个家庭协调的V2R基因：大约一半的基础Vsns coExpress VMN2R1家庭c具有家庭A8-10，B或D的单个V2R基因（'C1型V2RS'），另一半将VMN2R2通过Family C的单一V2R基因（'C2）（'C2'（'C2）类型v2rs'）。来自两个家庭的V2RS的协调共用的监管机制仍然明确。在这里，我们在胚胎干细胞中靶向靶向VMN2R1中的两种小鼠菌株。这些突变导致表达给定C1类型V2R的VSN的数量的差分减小。没有VMN2R2至VMN2R7的补偿表达。在没有VMN2R1的情况下，VSN轴突在辅助嗅灯泡的适当区域中聚结。纳米串的基因表达分析揭示了跨越C1型V2RS的表达水平的差异和分级减少。 C2类型的V2RS的表达式级别没有变化，有两个例外，我们被重新分类为C1类型。因此，对于给定的C2型V2R，似乎存在基因选择的固定概率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号