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首页> 外文期刊>The European Journal of Neuroscience >In vitro In vitro differentiation of neural stem cells derived from human olfactory bulb into dopaminergic‐like neurons
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In vitro In vitro differentiation of neural stem cells derived from human olfactory bulb into dopaminergic‐like neurons

机译：在体外的体外分化从人嗅灯泡中的神经干细胞进入多巴胺能物质的神经元

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Abstract This study describes a new accessible source of neuronal stem cells that can be used in Parkinson's disease cell transplant. The human olfactory bulb contains neural stem cells (NSC s) that are responsible for neurogenesis in the brain and the replacement of damaged cellular components throughout life.NSC s are capable of differentiating into neuronal and glial cells. We isolatedNSC s from the olfactory bulb of braindeath donors and differentiated them into dopaminergic neurons. The olfactory bulb tissues obtained were cultured in Dulbecco's modified Eagle's medium/nutrient mixture F12, B27 supplemented withbasic fibroblast growth factor ,epidermal growth factor andleukemia inhibitory factor . TheNSC s and proliferation markers were assessed. The multipotentiality of olfactory bulbNSC s was demonstrated by their capacity to differentiate into neurons, oligodendrocytes and astrocytes. To generate dopaminergic neurons, olfactory bulbNSC s were differentiated in neurobasal medium, supplemented with B27, and treated withsonic hedgehog , fibroblast growth factor 8 and glial cellderived neurotrophic factor from the 7th to the 21st day, followed by detection of dopaminergic neuronal markers including tyrosine hydroxylase and aromaticl amino acid decarboxylase. The cells were expanded, established in continuous cell lines and differentiated into the two classical neuronal phenotypes. The percentage of copositive cells (microtubuleassociated protein 2 and tyrosine hydroxylase; aromatic lamino acid decarboxylase andtyrosine hydroxylase ) in the treated cells was significantly higher than in the untreated cells. These results illustrate the existence of multipotentNSC s in the adult human olfactory bulb that are capable of differentiating toward putative dopaminergic neurons in the presence of trophic factors. Taken together, our data encourage further investigations of the possible use of olfactory bulbNSC s as a promising cellbased therapeutic strategy for Parkinson's disease.
机译：摘要本研究描述了可用于帕金森氏病细胞移植的新型无障碍源。人嗅灯泡含有神经干细胞（NSC S），其负责大脑中的神经发生，并且在整个寿命中替换受损的细胞组分。NSC S能够区分为神经元和胶质细胞。我们从脑膜炎料的嗅灯泡中脱离，并将它们分化为多巴胺能神经元。获得的嗅灯泡组织在Dulbecco的改性鹰的培养基/营养混合物F12，B27中培养，补充有吸收性成纤维细胞生长因子，表皮生长因子和细胞抑制因子。评估瑟斯C和增殖标志物。通过它们的能力来阐明嗅鳞茎的多电位性，以区分为神经元，少突胶细胞和星形胶质细胞。为了产生多巴胺能神经元，嗅到血糖培养基中的嗅疱疹，并在第7天至第21天中用刺猬刺猬，成纤维细胞生长因子8和胶质细胞的神经营养因子进行处理，然后检测包括酪氨酸羟化酶的多巴胺能神经元标记物和芳族氨基酸脱羧酶。将细胞扩增，在连续细胞系中建立并分化为两种典型的神经元表型。经处理细胞中的连体细胞（微管酶分子蛋白2和酪氨酸羟基化酶和幼料氨酸羟基化酶）的百分比显着高于未处理的细胞。这些结果说明了在成年人嗅灯泡中的多恒尼蛋白酶的存在能够在营养因子存在下朝向推定的多巴胺能神经元区分。我们的数据携带，我们的数据促进进一步调查可能使用嗅斗标作为帕金森病的有前途的蜂窝治疗策略。

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