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首页> 外文期刊>The European Journal of Neuroscience >Developmental origin of long‐range neurons in the superficial dorsal spinal cord
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Developmental origin of long‐range neurons in the superficial dorsal spinal cord

机译：浅层脊髓中远程神经元的发育起源

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Abstract The dorsal spinal cord, which is essential for somatosensory transmission, comprises a heterogeneous population of neurons with distinct axonal lengths and projection patterns. Although the developmental origin of localcircuit interneurons in the dorsal spinal cord has been well characterized, that of longrange neurons extending axons over a long distance such as supraspinal projection neurons and propriospinal neurons is largely unknown. In this study, we performed birthdate and lineage analyses of these longrange neurons in the mouse dorsal spinal cord. Unilateral injection of a retrograde neuronal tracer, cholera toxin B, into the brain or spinal cord efficiently labeled supraspinal projection neurons localized in Rexed's lamina I and the dorsolateral funiculus (DLF ) and longrange propriospinal neurons localized in theDLF , all of which had ipsi and contralaterally projecting populations. Most of these neurons were born between E9.5 and E10.5, much earlier than in the case of the neurogenesis of localcircuit neurons. Lineage analysis using an Lbx1Cre mouse line demonstrated that most longrange neurons were derived from Lbx1positive neuronal progenitors, except in the case of the contralaterally projecting propriospinal neurons. Characterization of their neurotransmitter identity revealed that almost all of the supraspinal projection neurons were excitatory, whereas the longrange propriospinal neurons comprised both excitatory and inhibitory populations. These results suggest that the supraspinal projection neurons were derived from the earlyborndI 5 progenitor domain and that the longrange propriospinal ones arose from several earlyborn progenitor domains.
机译：摘要胸脊髓，对于躯体感应透射至关重要，包括具有不同轴突长度和投影图案的异质神经元群。虽然背脊帘线中局部梗塞的型血管间的发育起源已经很好地表征，但是孤独的神经元在长距离延伸轴突，例如袋子突起神经元和ProprioStophininal神经元在很大程度上是未知的。在这项研究中，我们在小鼠背部脊髓中进行了这些Longrange神经元的出生金属和谱系分析。单侧注射逆行神经元示踪剂，霍乱毒素B，进入脑或脊髓的脑部或脊髓，有效地标记为克莱克德的薄膜I和沉淀的脊柱型菌株（DLSOLATAL Funiculus（DLF）和Longrange ProprioSto术神经元，所有这些都具有IPSI和对侧的所有内容投射人口。这些神经元的大多数均在E9.5和E10.5之间出生，比在局部梗塞神经元神经发生的情况下。使用LBX1CRE鼠标线的谱系分析证明了大多数Longrange神经元来自LBX1阳性神经元祖细胞，除了对侧突出的ProprioSto脊髓神经元的情况。其神经递质标识的表征揭示了几乎所有的袋子突起神经元是兴奋性的，而孤独的血清神经元均包含兴奋性和抑制性群体。这些结果表明，求袋突起神经元衍生自早期的祖先域，并且孤独的ProprioStoStoStoStophero血管源来自几种早期的祖域域。

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