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首页> 外文期刊>The European Journal of Neuroscience >Altered transcription of glutamatergic and glycinergic receptors in spinal cord dorsal horn following spinal cord transection is minimally affected by passive exercise of the hindlimbs
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Altered transcription of glutamatergic and glycinergic receptors in spinal cord dorsal horn following spinal cord transection is minimally affected by passive exercise of the hindlimbs

机译：在脊髓横划线后脊髓背角在脊髓背角中改变的谷氨酸和甘油酸受体转录最小受到后肢的被动运动的影响

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Abstract Gene expression is altered following a spinal transection (ST x) in both motor and sensory systems. Exercise has been shown to influence gene expression in both systems postST x. Gene expression alterations have also been shown in the dorsal root ganglia and nociceptive laminae of the spinal cord following either an incomplete spinal cord injury (SCI ) or a contusiveSCI . However, the effect ofST x and exercise on gene expression in spinal cord laminae IIII has not fully been examined. Therefore, the purpose of this study was to determine whether gene expression in laminae IIII is altered followingST x and determine whether superimposed passive exercise of the hindlimbs would influence gene expression postST x in laminae IIII . Laser capture microdissection was used to selectively harvest laminae IIII of lumbar spinal cord sections, and quantitativeRT PCR was used to examine relative expression of 23 selected genes in samples collected from control,ST x andST x plus exercise rats. We demonstrate that postST x, gene expression for metabotropic glutamate receptors 1, 5 and 8 were upregulated, whereas ionotropic glutamatergic receptor (Glur2) and glycinergic subunitGLRA 1 expression was downregulated. Daily exercise attenuated the downregulation of Glur2 gene expression in laminae IIII . Our results demonstrate that in aST x model, gene expression is altered in laminae IIII and that although passive exercise influences gene expression in both the motor and sensory systems, it had a minimal effect on gene expression in laminae IIII postST x.
机译：摘要在电动机和感官系统中的脊柱横截面（ST X）之后改变了基因表达。已经证明了运动术后X的两个系统中的基因表达。在不完全脊髓损伤（SCI）或Contusivesci之后，基因表达改变也已在脊髓的背根神经节和脊髓的伤害薄膜中显示。然而，X和锻炼对脊髓薄层IIII中的基因表达的影响尚未完全研究。因此，本研究的目的是确定薄层IIII中的基因表达是否改变了后X并确定了后肢的叠加被动运动是否会影响薄层IIII的基因表达胸部X。激光捕获微量碎石用于选择性地收获腰椎脊髓切片的椎板IIII，并且使用Quantitativert PCR来检查从对照中收集的样品中的23个选定基因的相对表达。我们证明胸部X，代购酶促谷氨酸受体1,5和8的基因表达，而离子孔谷氨酸谷氨酸受体（Glur2）和甘油池能亚群根1表达下调。日常运动减弱了薄膜IIII中GluR2基因表达的下调。我们的结果表明，在AST X模型中，基因表达在Laminae IIII中改变，尽管被动运动会影响电动机和感官系统中的基因表达，但它对Laminae IIII PTERST X的基因表达具有最小的影响。

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