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首页> 外文期刊>The European Journal of Neuroscience >Daily oscillation of odorant detection in rat olfactory epithelium
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Daily oscillation of odorant detection in rat olfactory epithelium

机译：大鼠嗅觉上皮的每日振动性检测

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Abstract Most of biological variables follow a daily rhythm. It holds true as well for sensory capacities as two decades of research have demonstrated that the odorant induced activity in the olfactory bulbs oscillates during the day. Olfactory bulbs are the first central nervous system structures, which receive inputs from the olfactory neurons located in the nose olfactory epithelium in vertebrates. So far, data on variation in odorant detection in the olfactory epithelium throughout the day are missing. Using electroolfactogram recordings in rats housed under daily light and dark cycles, we found that the olfactory epithelium responsiveness varies during the day with a maximum in the beginning of the light phase. This fluctuation was consistent with cycling of transduction pathway gene expression in the olfactory epithelium examined byqPCR . It was also consistent with the levels of two transduction pathway proteins (olfactorytype G protein and adenylyl cyclaseIII ) examined by western blot. Daily variations were also observed at the level of olfactory sensory neurons responses recorded by patchclamp. To rule out a potential effect of the feeding status of the animal, we examined the variation in odorant response in starved animals during the day. We observed a similar pattern toad libidum fed animals. Taken together, our results reveal that the olfactory epithelium sensitivity varies during the day in part due to modulation of the very first step of odorant detection.
机译：摘要大多数生物变量遵循每日节奏。对于Sensory能力而言，由于二十几年的研究表明，感觉能力证明了在白天嗅灯泡中的气味诱导的活性。嗅灯泡是第一种中枢神经系统结构，其从位于脊椎动物的鼻嗅性上皮内的嗅觉神经元接收输入。到目前为止，缺少整天嗅觉上皮内的气味检测的变化数据。使用在日常光线和黑暗循环的大鼠中使用电池从属图记录，我们发现嗅觉上皮响应性在当天在光相开始时变化。这种波动与嗅觉上皮中的转导途径基因表达的循环一致，检查通过QPCR。它还与Western印迹检查的两种转导途径蛋白（嗅觉型蛋白和腺苷酸环样胺）的水平一致。在Cappclamp记录的嗅觉感官神经元响应的水平下也观察到每日变化。为了排除动物喂养状态的潜在效果，我们在白天检查了饥饿的动物中的气味反应的变化。我们观察了类似的模式蟾蜍喂养动物。我们的结果表明，由于调制了气味检测的第一步，嗅觉上皮敏感性在一天内变化。

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