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首页> 外文期刊>The European Journal of Neuroscience >Metabotropic glutamate receptor 1-mediated calcium mobilization in the neonatal hippocampal marginal zone
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Metabotropic glutamate receptor 1-mediated calcium mobilization in the neonatal hippocampal marginal zone

机译：代谢谷氨酸受体1介导在新生儿海马边际区域中的钙动员

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The hippocampal marginal zone contains CajalRetzius (C-R) cells and participates in the regulation of cortical development. Two subtypes of group I metabotropic glutamate receptors (mGluRs), mGluR1 and mGluR5, are found in the central nervous system and are considered to regulate neuronal excitability. The release of Ca2+ from intracellular stores is thought to be a main consequence of activation of these receptor subtypes. In hippocampal C-R cells, the expression of mGluR1 has been showed using immunohistochemical techniques, but its function has not been elucidated. In this study, Ca2+ mobilization through mGluR1 activation was demonstrated in the neonatal rat hippocampus. In marginal zone C-R cells, intracellular Ca2+ elevation was detected by fluorescence imaging after the application of a group I mGluR-specific agonist. This response was prevented by application of an mGluR1 antagonist but was not changed by application of an mGluR5 antagonist. The intracellular Ca2+ elevation induced by mGluR1 activation was still observed in Ca2+-free perfusate, indicating the release of Ca2+ from intracellular stores. ?Aminobutyric acid and ionotropic glutamate receptor-mediated intracellular Ca2+ elevation was also detected in mGluR1-possessing neurons, although the former was much smaller than that mediated by mGluR1. These results indicate that mGluR1 is functionally expressed in C-R cells in the neonatal marginal zone and regulates cell function through the elevation of intracellular Ca2+. ?2018 Federation of European Neuroscience Societies and John Wiley & Sons Ltd
机译：海马边缘区含有Cajalretzius（C-R）细胞，并参与皮质发育的调节。在中枢神经系统中发现了两组代谢醇类谷氨酸受体（MGLURURS），MGLUR1和MGLUR5的两种亚型，并且被认为是调节神经元兴奋性。从细胞内储存的Ca2 +的释放被认为是激活这些受体亚型的主要结果。在海马C-R细胞中，使用免疫组织化学技术表达MGLUR1的表达，但其功能尚未阐明。在本研究中，在新生大鼠海马中证明了通过MGLUR1活化的CA2 +动员。在边缘区C-R细胞中，通过在施用Imlur特异性激动剂后通过荧光成像检测细胞内Ca2 +升高。通过施用MgluR1拮抗剂来防止这种反应，但未通过施用MgluR5拮抗剂而改变。在Ca2 + -Free灌注液中仍观察到MGLUR1活化诱导的细胞内Ca2 +升降液，表明来自细胞内储备的Ca2 +释放。氨基丁酸和离子型谷氨酸受体介导的细胞内Ca2 +升高在MgluR1 - 具有神经元中，尽管前者比MgluR1介导的那么小。这些结果表明，MGLUR1在新生儿边缘区的C-R细胞中在C-R细胞中表达，并通过细胞内Ca2 +的升高调节细胞功能。 ？2018年欧洲神经科学协会联合会和约翰瓦里和儿子有限公司

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